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RAIDD (human) polyclonal antibody (AT100)

 
ALX-210-915-C050 50 µg 205.00 USD
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Product Specification

Alternative Name:CRADD, Caspase and RIP adaptor with death domain, RIP-associated protein with a death domain
 
Host:Rabbit
 
Immunogen:Recombinant human RAIDD (aa 1-199).
 
UniProt ID:P78560
 
Species reactivity:Human
 
Applications:IP, WB
 
Recommended Dilutions/Conditions:Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Affinity purified.
 
Formulation:Liquid. In PBS containing 0.02% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Scientific Background:RAIDD (RIP-associated ICH-1 homologous protein with a death domain) is an adaptor molecule that mediates the action of cysteine proteases involved in apoptosis. In human cell lines, the caspase-2 adaptor RAIDD interacts selectively with caspase-2 through its caspase recruitment domain (CARD) and leads to caspase-2-dependent death. RAIDD overexpression induced caspase-2, CARD- and caspase-9-dependent apoptosis of PC12 cells and sympathetic neurons. Apoptosis correlated with the formation of discrete perinuclear aggregates. Both death and aggregates required the expression of full-length RAIDD. It has been reported that activation of caspase-2 occurs in a complex that contains the death domain–containing protein PIDD, whose expression is induced by p53, and the adaptor protein RAIDD.
 
210-915-new
Figure: Detection of endogenous RAIDD (human) in HEK293T cells by the PAb to RAIDD (AT100) (Prod. No. ALX-210-915).Method: Total cell extracts (50µg) were run on a SDS-gel, and revealed with anti-RAIDD antibody (AT100, 1:1000, affinity purified antibody, in 5% milk powder) for 1 hour at RT followed by incubation with HRP-coupled anti-rabbit secondary antibody (1:4’000). The arrow indicates endogenous human RAIDD.
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General Literature References

RAIDD aggregation facilitates apoptotic death of PC12 cells and sympathetic neurons: O. Jabado, et al.; Cell Death Differ. 11, 618 (2004), Abstract;
The PIDDosome, a protein complex implicated in activation of caspase-2 in response to genotoxic stress: A. Tinel and J. Tschopp; Science 304, 843 (2004), Abstract;

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