Product Details
Alternative Name: | CRADD, Caspase and RIP adaptor with death domain, RIP-associated protein with a death domain |
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Host: | Rabbit |
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Immunogen: | Recombinant human RAIDD (aa 1-199). |
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UniProt ID: | P78560 |
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Source: | Purified from rabbit serum. |
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Species reactivity: | Human
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Applications: | IP, WB
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Recommended Dilutions/Conditions: | Western Blot (1:1,000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Purity Detail: | Affinity purified. |
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Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Blue Ice |
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Short Term Storage: | +4°C |
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Long Term Storage: | -20°C |
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Scientific Background: | RAIDD (RIP-associated ICH-1 homologous protein with a death domain) is an adaptor molecule that mediates the action of cysteine proteases involved in apoptosis. In human cell lines, the caspase-2 adaptor RAIDD interacts selectively with caspase-2 through its caspase recruitment domain (CARD) and leads to caspase-2-dependent death. RAIDD overexpression induced caspase-2, CARD- and caspase-9-dependent apoptosis of PC12 cells and sympathetic neurons. Apoptosis correlated with the formation of discrete perinuclear aggregates. Both death and aggregates required the expression of full-length RAIDD. It has been reported that activation of caspase-2 occurs in a complex that contains the death domain–containing protein PIDD, whose expression is induced by p53, and the adaptor protein RAIDD. |
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Regulatory Status: | RUO - Research Use Only |
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Figure: Detection of endogenous RAIDD (human) in HEK293T cells by the PAb to RAIDD (AT100) (Prod. No.
ALX-210-915).
Method: Total cell extracts (50µg) were run on a SDS-gel, and revealed with anti-RAIDD antibody (AT100, 1:1000, affinity purified antibody, in 5% milk powder) for 1 hour at RT followed by incubation with HRP-coupled anti-rabbit secondary antibody (1:4’000). The arrow indicates endogenous human RAIDD."
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General Literature References
RAIDD aggregation facilitates apoptotic death of PC12 cells and sympathetic neurons: O. Jabado, et al.; Cell Death Differ.
11, 618 (2004),
Abstract;
The PIDDosome, a protein complex implicated in activation of caspase-2 in response to genotoxic stress: A. Tinel and J. Tschopp; Science
304, 843 (2004),
Abstract;