Product Specification
| Alternative Name: | Myeloid differentiation primary response protein |
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| Host: | Rabbit |
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| Immunogen: | Recombinant human MyD88 (myeloid differentiation primary response protein) (aa 1-172). |
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| UniProt ID: | Q99836 |
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| Source: | Whole rabbit serum. |
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| Species reactivity: | Human, Mouse
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| Specificity: | Recognizes the death domain of MyD88. |
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| Applications: | WB
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| Recommended Dilutions/Conditions: | Western Blot (1:5,000-1:8,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Formulation: | Liquid. Neat serum. |
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| Use/Stability: | Stable for at least 6 months after receipt when stored at +4°C undiluted. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice Not Frozen |
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| Long Term Storage: | +4°C |
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Figure: Detection of endogenous MyD88 in mouse EL-4 and human HEK 293T cells.
Method: Cell extracts from EL-4 (1) or 293T cells (2) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with the antiserum to MyD88 at a dilution of 1:8000. Proteins were visualized using a peroxidase-conjugated antibody to rabbit IgG and a chemiluminescence detection system.
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Product Literature References
Heme oxygenase-1 protects rat liver against warm ischemia/reperfusion injury via TLR2/TLR4-triggered signaling pathways: H.F. Huang, et al.; World J. Gastroenterol.
21, 2937 (2015),
Application(s): Western Blotting,
Abstract;
Full Text
Roles of toll-like receptors in C-C chemokine production by renal tubular epithelial cells: N. Tsuboi, et al.; J. Immunol.
169, 2026 (2002),
Abstract;
Tollip, a new component of the IL-1RI pathway, links IRAK to the IL-1 receptor: K. Burns, et al.; Nat. Cell Biol.
2, 346 (2000),
Abstract;
General Literature References
The apoptotic signaling pathway activated by toll-like receptor-2: A.O. Aliprantis, et al.; EMBO J.
19, 3325 (2000),
Abstract;
Full Text
Unresponsiveness of MyD88-deficient mice to endotoxin: T. Kawai, et al.; Immunity
11, 115 (1999),
Abstract;
MyD88 is an adaptor protein in the hToll/IL-1 receptor family signaling pathways: R. Medzhitov, et al.; Mol. Cell
2, 253 (1998),
Abstract;
MyD88, an adapter protein involved in interleukin-1 signaling : K. Burns, et al.; J. Biol. Chem.
273, 12203 (1998),
Abstract;
Full Text
Targeted disruption of the MyD88 gene results in loss of IL-1- and IL- 18-mediated function : O. Adachi, et al.; Immunity
9, 143 (1998),
Abstract;
IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL- 1 signaling : M. Muzio, et al.; Science
278, 1612 (1997),
Abstract;
