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MyD88 polyclonal antibody

ALX-210-900-R100 100 µl 370.00 USD
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Product Specification

Alternative Name:Myeloid differentiation primary response protein
Immunogen:Recombinant human MyD88 (myeloid differentiation primary response protein) (aa 1-172).
UniProt ID:Q99836
Species reactivity:Human, Mouse
Specificity:Recognizes the death domain of MyD88.
Recommended Dilutions/Conditions:Western Blot (1:5,000-1:8,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Formulation:Liquid. Neat serum.
Use/Stability:Stable for at least 6 months after receipt when stored at +4°C undiluted.
Handling:Avoid freeze/thaw cycles.
Shipping:Blue Ice Not Frozen
Long Term Storage:+4°C
Figure: Detection of endogenous MyD88 in mouse EL-4 and human HEK 293T cells.
Method: Cell extracts from EL-4 (1) or 293T cells (2) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with the antiserum to MyD88 at a dilution of 1:8000. Proteins were visualized using a peroxidase-conjugated antibody to rabbit IgG and a chemiluminescence detection system.
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Product Literature References

Heme oxygenase-1 protects rat liver against warm ischemia/reperfusion injury via TLR2/TLR4-triggered signaling pathways: H.F. Huang, et al.; World J. Gastroenterol. 21, 2937 (2015), Application(s): Western Blotting, Abstract; Full Text
Roles of toll-like receptors in C-C chemokine production by renal tubular epithelial cells: N. Tsuboi, et al.; J. Immunol. 169, 2026 (2002), Abstract;
Tollip, a new component of the IL-1RI pathway, links IRAK to the IL-1 receptor: K. Burns, et al.; Nat. Cell Biol. 2, 346 (2000), Abstract;

General Literature References

The apoptotic signaling pathway activated by toll-like receptor-2: A.O. Aliprantis, et al.; EMBO J. 19, 3325 (2000), Abstract; Full Text
Unresponsiveness of MyD88-deficient mice to endotoxin: T. Kawai, et al.; Immunity 11, 115 (1999), Abstract;
MyD88 is an adaptor protein in the hToll/IL-1 receptor family signaling pathways: R. Medzhitov, et al.; Mol. Cell 2, 253 (1998), Abstract;
MyD88, an adapter protein involved in interleukin-1 signaling : K. Burns, et al.; J. Biol. Chem. 273, 12203 (1998), Abstract; Full Text
Targeted disruption of the MyD88 gene results in loss of IL-1- and IL- 18-mediated function : O. Adachi, et al.; Immunity 9, 143 (1998), Abstract;
IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL- 1 signaling  : M. Muzio, et al.; Science 278, 1612 (1997), Abstract;

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