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VASP (human) polyclonal antibody

 
ALX-210-898-R100 100 µl 301.00 USD
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Product Specification

Alternative Name:Vasodilator stimulated phosphoprotein
 
Host:Rabbit
 
Immunogen:Recombinant human VASP (vasodilator stimulated phosphoprotein) fused to a His-tag.
 
UniProt ID:P50552
 
Species reactivity:Human
 
Specificity:Recognizes unphosphorylated as well as phosphorylated (Ser157) VASP.
 
Crossreactivity:Does not cross-react with mouse or rat VASP. For the detection of mouse VASP use Prod. No. ALX-210-880.
 
Applications:IF, WB
 
Recommended Dilutions/Conditions:Immunofluorescence of formaldehyde fixed cells (1:500-1:1000)
Western Blot (1:1500-1:3000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Positive Control:Human platelet protein (500µg), supplied at 5mg/ml in SDS sample buffer (100mM NaCl, 73mM TRIS/HCl pH 6.7, 10mM DTT, 8mM EDTA, 5% glycerol, 2% SDS, 10µg/ml Bromophenol Blue sodium salt).Use 5µl (25µg) per lane for Western blotting of tricine gels (13% acrylamide) or 15% Laemmli gels.
 
Formulation:Liquid. Contains 0.02% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Scientific Background:VASP (vasodilator stimulated phosphoprotein) is a proline-rich protein substrate of cAMP- and cGMP-dependent protein kinases. Phosphorylation of VASP at Ser-157 causes a mobility shift in SDS gel electrophoresis from 46 to 50 kDa, which has been used as a convenient marker to monitor cyclic nucleotide-dependent protein kinase activity. VASP is the founding member of the Ena-VASP protein family, comprising the Drosophila protein Enabled (Ena), its mouse homologue Mena (mammalian Enabled), and mouse EVL (Ena-VASP-like protein). With these proteins VASP shares a conserved overall domain organization:
a) the conserved N-terminal Ena-VASP homology domain 1 (EVH1), which mediates binding to a proline-rich motif
b) a more divergent proline-rich central domain (which is responsible for profilin binding)
c) a conserved C-terminal EVH2 domain.

VASP is expressed in a variety of mammalian cell types and tissues. In cultured cells, VASP is associated with focal adhesions, cell-cell contacts, microfilaments, and highly dynamic membrane regions. From in vitro binding data VASP has been suggested to link profilin to zyxin, vinculin, and the Listeria spp. surface protein ActA, respectively. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filament formation and the actin-dependent motility of intracellular bacterial pathogens.
 
210-898
Figure: Western blot comparing three different PAbs to VASP using 25µg human platelet protein. Lane 1: PAb to VASP (human) (Prod. N. ALX-210-898). Lane 2: PAb to VASP (M4) (Prod. N. ALX-210-725) (Not available anymore!). Lane 3: PAb to VASP (affinity purified) (Prod. N. ALX-210-880).
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210-898

Product Literature References

Cyclic nucleotide phosphodiesterase 1 regulates lysosome-dependent type I collagen protein degradation in vascular smooth muscle cells: Y. Cay, et al.; Arterioscler. Thromb. Vasc. Biol. 31, 616 (2011), Abstract; Full Text
Proteomics identifies thymidine phosphorylase as a key regulator of the angiogenic potential of colony-forming units and endothelial progenitor cell cultures: G. Pula, et al.; Circ. Res. 104, 32 (2009), Abstract; Full Text

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VASP polyclonal antibody (affinity purified) 

ICC, IP, WB | Print as PDF
 
ALX-210-880-C025 25 µg 430.00 USD
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