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VASP polyclonal antibody (affinity purified)

 
ALX-210-880-C025 25 µg 430.00 USD
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Product Specification

Alternative Name:Vasodilator stimulated phosphoprotein (affinity purified)
 
Host:Rabbit
 
Immunogen:Recombinant human VASP.
 
UniProt ID:P50552
 
Species reactivity:Human, Mouse
Porcine
 
Specificity:Detects both the 46kDa (Ser157 dephospho) and 50kDa (Ser157 phospho) forms of VASP.
 
Applications:ICC, IP, WB
 
Recommended Dilutions/Conditions:Immunocytochemistry (formaldehyde fixed cells, 1µg/ml, Immunoprecipitation (2µg/ml)
Western Blot (0.1µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Positive Control:Human platelet protein (500µg) (Prod. No. ALX-840-007), supplied at 5mg/ml in SDS sample buffer (100mM NaCl, 73mM TRIS/HCl pH 6.7, 10mM DTT, 8mM EDTA, 5% glycerol, 2% SDS, 10µg/ml Bromophenol Blue sodium salt).Use 5µl (25µg) per lane for Western blotting of tricine gels (13% acrylamide) or 15% Laemmli gels.
 
Purity Detail:Affinity purified.
 
Formulation:Liquid. In PBS containing 1mg/ml BSA and 0.02% sodium azide.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Scientific Background:VASP (vasodilator stimulated phosphoprotein) is a proline-rich protein substrate of cAMP- and cGMP-dependent protein kinases. Phosphorylation of VASP at Ser-157 causes a mobility shift in SDS gel electrophoresis from 46 to 50 kDa, which has been used as a convenient marker to monitor cyclic nucleotide-dependent protein kinase activity. VASP is the founding member of the Ena-VASP protein family, comprising the Drosophila protein Enabled (Ena), its mouse homologue Mena (mammalian Enabled), and mouse EVL (Ena-VASP-like protein). With these proteins VASP shares a conserved overall domain organization:
a) the conserved N-terminal Ena-VASP homology domain 1 (EVH1), which mediates binding to a proline-rich motif
b) a more divergent proline-rich central domain (which is responsible for profilin binding)
c) a conserved C-terminal EVH2 domain.

VASP is expressed in a variety of mammalian cell types and tissues. In cultured cells, VASP is associated with focal adhesions, cell-cell contacts, microfilaments, and highly dynamic membrane regions. From in vitro binding data VASP has been suggested to link profilin to zyxin, vinculin, and the Listeria spp. surface protein ActA, respectively. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filament formation and the actin-dependent motility of intracellular bacterial pathogens.
 

Product Literature References

VASP phosphorylation at serine239 regulates the effects of NO on smooth muscle cell invasion and contraction of collagen: O.D. Defawe, et al.; J. Cell. Physiol. 222, 230 (2010), Abstract; Full Text
The nitration of platelet vasodilator stimulated phosphoprotein following exposure to low concentrations of hydrogen peroxide: M. Sabetkar, et al.; Platelets 19, 282 (2008), Abstract;
Adapter protein SH2-Bbeta stimulates actin-based motility of Listeria monocytogenes in a vasodilator-stimulated phosphoprotein (VASP)-dependent fashion: M. Diakonova, et al.; Infect. Immun. 75, 3581 (2007), Abstract;
Ena/VASP proteins mediate repulsion from ephrin ligands: I.R. Evans, et al.; J. Cell Sci. 120, 289 (2007), Abstract; Full Text
PtdIns(4,5)P-restricted plasma membrane localization of FAN is involved in TNF-induced actin reorganization: D. Haubert, et al.; EMBO J. 26, 3308 (2007), Abstract; Full Text
Vasodilator-stimulated phosphoprotein (VASP) is phosphorylated on Ser157 by protein kinase C-dependent and -independent mechanisms in thrombin-stimulated human platelets: J.K. Wentworth, et al.; Biochem. J. 393, 555 (2006), Abstract; Full Text
A role for VASP in RhoA-Diaphanous signalling to actin dynamics and SRF activity: R. Grosse, et al.; EMBO J. 22, 3050 (2003), Abstract;

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