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Toll-like receptor 7 polyclonal antibody

 
ALX-210-874-C100 100 µg 356.00 USD
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Product Specification

Alternative Name:TLR7
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to aa 706-728 of human TLR7 (Toll-like receptor 7).
 
UniProt ID:Q9NYK1
 
Species reactivity:Human, Mouse, Rat
 
Applications:Flow Cytometry, ICC, IHC (FS), IHC (PS), IP, WB
 
Recommended Dilutions/Conditions:Flow Cytometry (intracellular, 0.5-5µg/106 cells)
Immunohistochemistry (paraffin sections, 5-10µg/ml)
Western Blot (1-3µg/ml)
 
Application Notes:Flow Cytometry: suitable for cell surface and intracellular FC.
Western Blot: in human Ramos cells, additional bands are seen
 
Purity Detail:Protein G-affinity purified.
 
Formulation:Liquid. In PBS containing 0.05% BSA and 0.05% sodium azide.
 
Use/Stability:Stable for 6 months when stored at +4°C.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
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Figure 1: Western blot analysis of TLR7 in Raw cell lysate using Prod. No. ALX-210-874 at 1µg/ml.
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Figure 2: Intracellular staining by FACS analysis of TLR7 in Ramos cells (A) and Raw cells (B) using Prod. No. ALX-210-874 at 2µg/ml. Histogram C shows the rabbit IgG isotype control.
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Figure 3: Immunohistochemical analysis of human colon tissue using PAb to Toll-like Receptor 7 (Prod. No. ALX-210-874) and an isotype control (bottom) at 5μg/ml.
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Product Literature References

Antitumor effects of imidazoquinolines in urothelial cell carcinoma of the bladder: E.B. Smith, et al.; J. Urol. 177, 2347 (2007), Abstract;
Members of the Toll-like receptor family of innate immunity pattern-recognition receptors are abundant in the male rat reproductive tract: M.A. Palladino, et al.; Biol. Reprod. 76, 958 (2007), Abstract;
The interaction between the ER membrane protein UNC93B and TLR3, 7, and 9 is crucial for TLR signaling: M.M. Brinkmann, et al.; J. Cell Biol. 177, 265 (2007), Abstract; Full Text
Expression and regulation of Toll-like receptors in lupus-like immune complex glomerulonephritis of MRL-Fas(lpr) mice: P.S. Patole, et al.; Nephrol. Dial. Transplant. 21, 3062 (2006), Abstract;
Toll-like receptor-7 modulates immune complex glomerulonephritis: R.D. Pawar, et al.; J. Am. Soc. Nephrol. 17, 141 (2006), Abstract; Full Text
Expression of mRNA and proteins for toll-like receptors, associated molecules, defensins and LL-37 by SRIK-NKL, a CD8+ NK/T cell line: M.D. Srivastava and B.I. Srivastava; Leuk. Res. 29, 813 (2005), Abstract;
Inhibition of neutrophil apoptosis by TLR agonists in whole blood: involvement of the phosphoinositide 3-kinase/Akt and NF-kappaB signaling pathways, leading to increased levels of Mcl-1, A1, and phosphorylated Bad: S. Francois, et al.; J. Immunol. 174, 3633 (2005), Abstract;
TLR7/8-mediated activation of human NK cells results in accessory cell-dependent IFN-gamma production: O.M. Hart, et al.; J. Immunol. 175, 1636 (2005), Abstract;

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