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Caspase-9 (human) polyclonal antibody

 
ALX-210-838-R100 100 µl 367.00 USD
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Product Specification

Host:Rabbit
 
Immunogen:Recombinant human full-length caspase-9.
 
UniProt ID:P55211
 
Species reactivity:Human
 
Specificity:Recognizes procaspase-9 and the p37/p35 cleavage products of activated caspase-9.
 
Applications:IP, WB
 
Application Notes:Recommended for the detection of endogenous caspase-9 activation.
 
Formulation:Liquid. Antiserum containing 0.02% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
210-838-n1
Figure: (A) Schematic structure of caspase-9 and its cleavage products as detected by anti-caspase-9.
210-838-n2
Figure: (B) Detection of caspase-9 processing during apoptosis. The antiserum detects procaspase-9 (46kDa) and the intermediate cleavage products of 37kDa and 35 kDa. No cross-reactivity with other caspases is observed.Method: Jurkat cells were treated with 2µM staurosporine (Prod. No. ALX-380-014). After 4 hours cell lysates were prepared and separated by SDS-Page (2x106 cells/lane) under reducing conditions. Proteins were immunoblotted with rabbit anti-caspase-9 (1:1’000). Following incubation with peroxidase-conjugated secondary antibodies caspase-9 processing was detected by enhanced chemoluminescent staining. The closed arrow indicates procaspase-9 (46kDa), which processed to the p35 and p37 fragments (open arrows).
210-838-n3
Figure: (C) Immunoprecipitation of caspase-9.
Method: Extracts of 1x107 Jurkat cells were subjected to immunoprecipitation with Protein G-sepharose in the presence of rabbit preimmune serum (PI control, lane 1) or the antiserum to caspase-9 (lane 2). Lane 3 demonstrates that the antiserum is able to completely deplete caspase-9 from cell lysates, as no caspase-9 was detected in the supernatants after precipititaion.
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Product Literature References

The prevention of spontaneous apoptosis of follicular lymphoma B cells by a follicular dendritic cell line: involvement of caspase-3, caspase-8 and c-FLIP: J.J. Goval, et al.; Haematologica 93, 1169 (2008), Application(s): WB using human follicular lymphoma lysate, Abstract; Full Text

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