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Caspase-1 polyclonal antibody

 
ALX-210-804-C100 100 µg 323.00 USD
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Product Specification

Alternative Name:IL-1β converting enzyme, Interleukin-1β converting enzyme, ICE
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to aa 224-242 (CEPH224KTSDSTFLVFMSHGIREG242) of human caspase-1.
 
UniProt ID:P29466
 
Species reactivity:Human, Mouse, Rat
 
Applications:ICC, IHC, IP, WB
 
Recommended Dilutions/Conditions:Immunocytochemistry (10-20µg/ml)
Immunohistochemistry (1:50)
Immunoprecipitation (4-8µg/ml)
Western Blot (0.5-4µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects bands of ~45kDa (procaspase-1) and ~20kDa (cleavage product) by Western blot.
 
Purity Detail:Epitope-affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
210-804
Figure: Western blot analysis of caspase-1 expression in rat liver tissue lysate using Caspase-1, pAb (Prod. No. ALX-210-804).
ALX-210-804 IHC
Immunohistochemistry staining of human lung tumor tissue using Caspase-1, pAb (Prod. no. ALX-210-804) at a 1:50 dilution showed positive staining in both cytoplasm and nucleus of lung epithelial cells. Images were taken at magnification of 20 x (A) and 63 x (B).
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210-804 ALX-210-804 IHC

Product Literature References

FANCA and FANCC modulate TLR and p38 MAPK-dependent expression of IL-1β in macrophages: M.R. Garbati, et al.; Blood 122, 3197 (2013), Abstract;

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