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CCR8 (human) (NT) polyclonal antibody

 
ALX-210-762-C200 200 µg 356.00 USD
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Product Specification

Alternative Name:C-C chemokine receptor 8, CD198
 
Host:Goat
 
Immunogen:Synthetic peptide corresponding to aa 12-36 (V12TDYYYPDIFSSPCDAELIQTNGKL36) of the N-terminal extracellular domain of human CCR8 (C-C chemokine receptor 8).
 
UniProt ID:P51685
 
Species reactivity:Human
 
Applications:Flow Cytometry, ICC, IHC (PS), WB, FUNC
 
Recommended Dilutions/Conditions:Flow Cytometry (2-5µl per 106 cells/50-100µl)
Immunocytochemistry (acetone fixed cells, 1:800)
Immunohistochemistry (paraffin sections, 1:250)
Western Blot (1:2,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Functional Application: Neutralizing; inhibits Lp(a) induced MCA in HUVECS.
 
Purity Detail:Epitope-affinity purified IgG.
 
Quality Control:Immunocytochemistry on human K562 cells and immunohistochemistry on human spleen tissue slices.
 
Formulation:Liquid. In PBS containing 1mg/ml BSA and 0.1% sodium azide.
 
Handling:For maximum product recovery after thawing, centrifuge the vial before opening the cap. Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Protocol:Immunohistochemistry on paraffin sections:
- Fix paraffin sections in 10% formalin.
- De-wax sections and block endogenous peroxide by incubating sections with 3% H2O2 for 15 min. at room temperature (RT).
- Wash sections with PBS and incubate with 2% normal rabbit serum at room temperature to block non-specific binding sites.
- Wash sections with PBS and incubate with primary antibody (anti-chemokine receptor) at recommended dilution for 60 min. at RT.
- Wash slides and incubate at RT with biotinylated rabbit anti-goat IgG H+L (Vector Labs) at 1:200 dilution for 30 min.
- Wash slides and incubate with HRP-Streptavidin (Vector Labs) 1:400 dilution for 30 min.
- Wash slides and incubate with DAB for 5-7 min. at RT.
- Wash slides with PBS and counter stain with hematoxylin.
 

Product Literature References

CCL16 activates an angiogenic program in vascular endothelial cells: M. Strasly, et al.; Blood 103, 40 (2004), Abstract;
The chemokine receptor CCR8 mediates human endothelial cell chemotaxis induced by I-309 and Kaposi sarcoma herpesvirus-encoded vMIP-I and by lipoprotein(a)-stimulated endothelial cell conditioned medium: N.S. Haque; Blood 97, 39 (2001), Abstract;
CC Chemokine I-309 is the Principal Monocyte Chemottractant Induced by Apolipoprotein(a) in Human Vascular Endothelial Cells: N.S. Haque; Circulation 102, 786 (2000), Abstract;
The CC chemokine I-309 inhibits CCR8-dependent infection by diverse HIV-1 strains: R. Horuk, et al.; J. Biol. Chem. 273, 386 (1998), Abstract; Full Text
Molecular cloning and chromosomal mapping of a novel human gene, ChemR1, expressed in T lymphocytes and polymorphonuclear cells and encoding a putative chemokine receptor: M. Samson, et al.; Eur. J. Immunol. 26, 3021 (1996), Abstract;

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