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Apurinic/apyrimidinic endonuclease polyclonal antibody

 
ALX-210-723-R100 100 µl 290.00 USD
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Product Specification

Alternative Name:APE1, APEX, HAP1, Ref-1
 
Host:Rabbit
 
Immunogen:Recombinant human APE1 (apurinic/apyrimidinic endonuclease).
 
UniProt ID:P27695
 
Species reactivity:Human, Mouse, Rat
 
Applications:ICC, IHC (FS), IHC (PS), WB
 
Application Notes:Detects a band of ~37kDa by Western blot.
Immunohistochemistry: Human and rat only.
 
Formulation:Liquid. 100µg/ml affinity-purified rabbit antiserum in PBS. Contains no preservatives.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Apurinic/apyrimidinic endonuclease (APEX, HAP1, Ref-1) is a multifunctional, bipartite enzyme that belongs to the class II AP endonucleases and that plays an important role in numerous, cellular functions, like repairing abasic sites (loss of purine or pyrimidine base) in DNA, regulating the redox state of other proteins that play roles in oxidative signalling, transcription factor regulation (Fos, Jun, NF-κB, Myb, HIF-1α, CREB, Pac), cell cycle control (p53) and apoptosis. APE1 initiates the DNA base excision repair (BER) by cleaving the DNA immediately adjacent to the 5' of an AP site to produce a hydroxyl group at the 3' terminus of an unmodified nucleotide upstream of the nick and a 5'-deoxyribose phosphate moiety downstream. The product of APE1 is further processed by DNA polymerase β to release 5'-deoxyribose phosphate with its intrinsic lyase activity. The nicked DNA is then sealed by DNA ligase I or DNA ligaseIII/XRCC1 to complete this repair process. In addition to the AP endonuclease activity, APE1 also possesses a 3'-5' DNA exonuclease activity, a 3'-phosphodiesterase activity, a 3'-phosphatase activity, and a RNase H activity. It has been shown that APE1 is capable of excision L-configuration deoxyribonucleoside analogs from the 3' termini of DNA. Human APE1 gene is located on chromosome 14q 11.2-12.
 

Product Literature References

Down-regulation of apurinic/apyrimidinic endonuclease expression is associated with the induction of apoptosis in differentiating myeloid leukemia cells: K.A. Robertson, et al.; Cell Growth Differ. 8, 443 (1997), Abstract;
Focal nuclear hepatocyte response to oxidative damage following low dose thioacetamide intoxication: G.A. Clawson, et al.; Carcinogenesis 18, 1663 (1997), Abstract;
The apurinic/apyrimidinic endonuclease (APE/ref-1) DNA repair enzyme is elevated in premalignant and malignant cervical cancer: Y. Xu, et al.; Anticancer Res. 17, 3713 (1997), Abstract;
Differential cellular and subcellular expression of the human multifunctional apurinic/apyrimidinic endonuclease (APE/ref-1) DNA repair enzyme: J.R. Duguid, et al.; Cancer Res. 55, 6097 (1995), Abstract;

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