Bcl-2, pAb

Concentration:	0.5mg/ml

Formulation:	Liquid. In PBS containing 0.09% sodium azide.

Immunogen:	Synthetic peptide corresponding to aa 20-34 of human Bcl-2.

Source/Host:	From rabbit.

Specificity:	Recognizes human, mouse and opossum Bcl-2. This antibody is particularly useful for the determination of Bcl-2 expression levels in species other than human.

Application:	Immunohistochemistry: Frozen sections: 5µg/ml; no pre-treatment needed. Paraffin sections: 2.5µg/ml; pressure cooker antigen retrieval is recommended. For both: DAB detection. Intestine or human lymph node can be used as positive control, brain tissue as negative control (only random cells will stain for Bcl-2 in brain tissue). Western Blot: 5µg/ml; ECL detection. MCF-7 cells can be used as a positive control, normal rabbit IgG as negative control. Titration is recommended for optimal results in individual systems.

Long Term Storage:	+4°C

Handling:	Do not freeze.