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CCR4 (mouse) polyclonal antibody

 
ALX-210-621-C200 200 µg 291.00 USD
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Product Specification

Alternative Name:C-C chemokine receptor 4, CD194
 
Host:Goat
 
Immunogen:Synthetic peptide corresponding to aa 8-26 (D8TTQDETVYNS
YYFYESMP26C) of the N-terminal extracellular domain of mouse CCR4 (C-C chemokine receptor 4).
 
UniProt ID:P51680
 
Species reactivity:Mouse
 
Crossreactivity:Does not cross-react with human CCR4.
 
Applications:ELISA, Flow Cytometry, ICC, IHC (PS), WB
 
Recommended Dilutions/Conditions:ELISA (1:100,000)
Flow Cytometry (1-3µl per 2x105 cells per 50µl)
Immunocytochemistry (1:500)
Immunohistochemistry (1:500)
Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Epitope-affinity purified F(ab`)2 fragment.
 
Quality Control:Immunocytochemistry on mouse spleen cells.
 
Formulation:Liquid. In PBS containing 1mg/ml BSA and 0.1% sodium azide.
 
Handling:For maximum product recovery after thawing, centrifuge the vial before opening the cap. Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Mouse CCR4 is the homolog of human CCR4 receptor (85% identical), binding macrophage inflammatory protein-1α (MIP-1α) and RANTES. Mouse CCR4 transcripts are expressed in the thymus and in T-cell lines.
 
210-621
Figure: Immunohistochemistry on paraffin section of mouse spleen with CCR4 (mouse), pAb (Prod. No. ALX-210-621).
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210-621

Product Literature References

CD4+CD25+ regulatory T cells in the small intestinal lamina propria show an effector/memory phenotype: Z. Guo, et al.; Int. Immunol. 20, 307 (2008), Application(s): Flow cytometry, Abstract;
CC-chemokine ligand 17 gene therapy induces tumor regression through augmentation of tumor-infiltrating immune cells in a murine model of preexisting CT26 colon carcinoma: N. Kanagawa, et al.; Int. J. Cancer 121, 2013 (2007), Application(s): Flow cytometry, Abstract;
IL-23 and IL-17 in the establishment of protective pulmonary CD4+ T cell responses after vaccination and during Mycobacterium tuberculosis challenge: S.A. Khader, at al.; Nat. Immunol. 8, 369 (2007), Application(s): Flow cytometry, Abstract;
Alloantigen-presenting plasmacytoid dendritic cells mediate tolerance to vascularized grafts: J.C. Ochando, et al.; Nat. Immunol. 7, 652 (2006), Application(s): Flow cytometry, Abstract;
Induction of surface CCR4 and its functionality in mouse Th2 cells is regulated differently during Th2 development: Y. Morimoto, et al.; J. Leukoc. Biol. 78, 753 (2005), Application(s): Flow cytometry, Abstract;
Role of CCR4 ligands, CCL17 and CCL22, during Schistosoma mansoni egg-induced pulmonary granuloma formation in mice: C. Jakubzick, et al.; Am. J. Pathol. 165, 1211 (2004), Application(s): Immunohistochemistry, Abstract; Full Text
Molecular cloning of murine CC CKR-4 and high affinity binding of chemokines to murine and human CC CKR-4: A. Hoogewerf, et al.; BBRC 218, 337 (1996), Abstract;

General Literature References

Molecular cloning of murine CC CKR-4 and high affinity binding of chemokines to murine and human CC CKR-4: A. Hoogewerf, et al.; BBRC 218, 337 (1996), Abstract;

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