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N-cadherin polyclonal antibody

 
ALX-210-618-C400 400 µg 722.00 USD
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Product Specification

Alternative Name:CD325, Cadherin-2, Neural cadherin
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to aa 808-827 of human N-cadherin.
 
UniProt ID:P19022
 
Species reactivity:Human, Mouse
Bovine, Chicken
 
Crossreactivity:Does not cross-react with human E- or P-cadherin.
 
Applications:IHC (FS), IHC (PS), WB
 
Recommended Dilutions/Conditions:Immunohistochemistry (frozen and paraffin sections, 5-10µg/ml)
Western Blot (5-10µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Does not inhibit N-cadherin dependent cell-cell contact.
 
Purity Detail:Protein A-affinity purified.
 
Formulation:Lyophilized from 10mM PBS, pH 7.4,  containing 1% BSA. Does not contain preservatives.
 
Reconstitution:Reconstitute with 200µl distilled water. TBS should be used for dilution as PBS may cause precipitation. Dilute with 20mM TBS, pH 7.4, containing 1% BSA and 0.1% sodium azide and 10mM calcium chloride. In case of immunohistochemistry on paraffin or frozen sections, Ca2+ is not necessary.
 
Use/Stability:Stable for at least 2 years after receipt when stored at +4°C. Reconstituted antibody is stable for 6 months when stored at +4°C and for 1 year when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:+4°C
 
210-618
Figure: Immunohistochemistry of mouse embryo (15 days) paraffin embedded tissues using PAb to N-cadherin (Prod. No. ALX-210-618).Method:Antigen : microwave treatment (3 min.×10 times) (10 mM citrate buffer, pH 6.0)Reaction of the primary antibody : 10 µg/ml, 1 hrDetection : Envision plus (Dako)Colorimetric substrate : DAB
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210-618

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Cadherin
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