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Polycomb 2 polyclonal antibody

 
ALX-210-570-C100 100 µg 420.00 USD
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Product Specification

Alternative Name:Pc2, CBX4
 
Host:Rabbit
 
Immunogen:Recombinant N-terminal part of human Pc2 (polycomb 2) (aa 1-228).
 
UniProt ID:O00257
 
Species reactivity:Human, Mouse
 
Applications:IP, WB
 
Recommended Dilutions/Conditions:Western Blot (2µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Protein A-affinity purified.
 
Formulation:Liquid. In PBS containing 0.1% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
210-570 1
Figure 1: Immunodetection of human Pc2 in nuclear extracts prepared from HEK 293 cells using PAb to Pc2 (Prod. No. ALX-210-570). Cells were transfected with hPc2 cDNA (right) or from the parental cells (left). The arrow shows migration of endogenous Pc2 protein.
210-570 2
Figure 2: Immunodetection of endogenous human Pc2 in nuclear extracts prepared from different cell lines using PAb to Pc2 (Prod. No. ALX-210-570). The arrow indicates a migration of putative full-length Pc2 protein. The non-specific cross-reacting bands are marked by the asterisks.
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210-570 1 210-570 2

General Literature References

Mechanism of histone lysine methyl transfer revealed by the structure of SET7/9-AdoMet: T. Kwon, et al.; EMBO J. 22, 292 (2003), Abstract;
Structure and catalytic mechanism of the human histone methyltransferase SET7/9: B. Xiao, et al.; Nature 421, 652 (2003), Abstract;
The polycomb protein Pc2 is a SUMO E3: M.H. Kagey, et al.; Cell 113, 127 (2003), Abstract;

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