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TetR polyclonal antibody

 
ALX-210-559-R100 100 µl 205.00 USD
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This antibody can also be excellently used in RNAi expression systems relying on TetR regulation, by verifying the TetR expression levels. Double-check your cell-line if it is suitable for inducible, tet-repressor dependent RNAi expression.

Product Specification

Alternative Name:Tetracycline repressor
 
Host:Rabbit
 
Immunogen:Recombinant TetR (tetracycline repressor).
 
UniProt ID:P04483 (E. coli)
 
Species reactivity:Species independent
 
Specificity:Recognizes the TetR portion of bait fusion protein in the yeast two-hybrid system.
 
Applications:WB
 
Recommended Dilutions/Conditions:Western Blot (1:500)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~37kDa by Western blot.
 
Formulation:Liquid. Neat serum in PBS containing 50% glycerol and traces of sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Scientific Background:TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to tetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites.
 
210-559
Figure: Western blot analysis of yeast lysate (~100µg whole cell protein per lane) of a yeast strain expressing the TetR domain fusion protein (+) or lysates of the yeast control strain (-)  using a 1:500 dilution of PAb to TetR (Prod. No. ALX-210-559), 10% SDS-PAGE, 30 seconds exposure.
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210-559

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