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PARP-1 (BRCT Domain) polyclonal antibody

 
ALX-210-540-R100 100 µl 215.00 USD
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Product Specification

Alternative Name:Poly(ADP-ribose) polymerase-1
 
Host:Rabbit
 
Immunogen:Recombinant human PARP-1 (BRCT domain) (aa 385-524) fused to a His-tag (Prod. No. ALX-201-255).
 
UniProt ID:P09874
 
Species reactivity:Human, Mouse
 
Applications:IP, WB
 
Recommended Dilutions/Conditions:Immunoprecipitation (1:200)
Western Blot (1:8,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Does not work for ICC.
 
Purity Detail:Ammonium sulfate precipitate.
 
Formulation:Liquid. In PBS containing 0.02% sodium azide.
 
Use/Stability:Stable for at least one year when stored at +4°C.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:+4°C
 
210-540 wb
Figure 1: Western blot analysis of endogenous and recombinant PARP-1 using PAb to PARP-1 (BRCT Domain) (Prod. No. ALX-210-540). Lane 1: Recombinant human His-tagged PARP-1 BRCT domain (Prod. No. ALX-201-255) (30ng). Lane 2: Hela 5x104 cells. Lane 3: MEFs 105 cells. Lane 4: HL60 105 cells. Lane 5: HL60 105 cells treated with etoposide for 4 hours. The antibody detects the 85kDa cleavage product.
210-540 ip
Figure 2: Immunoprecipitation of endogenous PARP-1 from HeLa cells using PAb to PARP-1 (BRCT Domain) (Prod. No. ALX-210-540) (1:200). Lane 1: PAb to PARP-1 (BRCT Domain). Lane 2: Pre-immuneserum.
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210-540 wb 210-540 ip

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PARP-1 (BRCT domain) (human), (recombinant) (His-tag) 

Produced in E. coli. Human PARP-1 (BRCT domain) (aa 385-524) is fused to a His-tag., ≥99% (SDS-PAGE) | Print as PDF
 
ALX-201-255-C020 20 µg 398.00 USD
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