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XRCC1 (human) polyclonal antibody

 
ALX-210-539-R100 100 µl 356.00 USD
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Product Specification

Alternative Name:X-ray repair cross-complementing group 1
 
Host:Rabbit
 
Immunogen:Recombinant human XRCC1 (X-ray repair cross-complementing group 1) fused to a His-tag.
 
UniProt ID:P18887
 
Species reactivity:Human
 
Applications:ICC, IP, WB
 
Recommended Dilutions/Conditions:Immunocytochemistry (1:1,000)
Immunoprecipitation (1:200)
Western Blot (1:2,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Formulation:Liquid. Antiserum containing 0.1% sodium azide.
 
Use/Stability:Stable for at least one year when stored at +4°C.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:+4°C
 
210-539 wb
Figure 1: Western blot analysis of endogenous and recombinant XRCC1 using PAb to XRCC1 (human) (Prod. No. ALX-210-539). Lane 1: HeLa cells. Lane 2: Recombinant human XRCC1 (10ng).
210-539 ip
Figure 2: Immunoprecipitation of endogenous XRCC1 from HeLa cells. Lane 1: PAb to XRCC1 (human) (Prod. No. ALX-210-304) (5µl). Lane 2: PAb to XRCC1 (human) (Prod. No. ALX-210-304) (10µl). Lane 3: PAb to XRCC1 (human) (Prod. No. ALX-210-539) (5µl). Lane 4: PAb to XRCC1 (human) (Prod. No. ALX-210-539) (10µl). Lane 5: Pre-immune serum.
210-539 icc
Figure 3: Immunocytochemistry of endogenous XRCC1 in HeLa cells using PAb to XRCC1 (human) (Prod. No. ALX-210-539) (1:1’000).
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210-539 wb 210-539 ip 210-539 icc

Product Literature References

Radiation-induced mitotic catastrophe in PARG-deficient cells: JC. Amé, et al.; J. Cell Sci. 122, 1990 (2009), Abstract;
XRCC1 is phosphorylated by DNA-dependent protein kinase in response to DNA damage: N. Levy, et al.; Nucl. Acids Res. 34, 32 (2006), Abstract; Full Text
XRCC1 is specifically associated with poly(ADP-ribose) polymerase and negatively regulates its activity following DNA damage: M. Masson, et al.; Mol. Cell. Biol. 18, 3563 (1998), Abstract; Full Text

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