Endothelin A receptor, pAb



ALX-210-507A-C250	250 µg	314.00 USD

Product Specification

Alternative Name:	ETA-receptor

Concentration:	1mg/ml

Purity Detail:	Epitope-affinity purified.

Formulation:	Liquid. In TRIS-citrate-phosphate, pH 7-8, containing 0.1% sodium azide.

Immunogen:	Synthetic peptide corresponding to aa 410-422 (Q⁴¹⁰EQNHNTERSSHK⁴²²) of C-terminal rat ET_A-receptor (endothelin A receptor).

Source/Host:	From sheep.

Specificity:	Recognizes an epitope on the cytoplasmic side of human, mouse and rat ET_A-receptor. Detects bands of 34-52kDa. Does not block the binding of endothelins to the receptor.

Application:	Immunohistochemistry (paraffin sections (4µg/ml)) Western Blot (10-20µg/ml using ECL)

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Handling:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Miscellaneous/General:	Endothelins are a family of peptides that are potent vasoconstrictors produced by mammalian vascular endothelial cells. The receptors for the endothelins are found on various tissues including, but not restricted to, lung and intestine.

Background / Technical Information:	Note: As this antibody is generated against the cytosolic portion of endothelin A receptor, permeabilization is required for immunochemical analysis.

Figure: Immunohistochemical staining of invasive ductal carcinoma (G2) using PAb to Endothelin A Receptor (Prod. No. ALX-210-507A). Expression can be seen in the cytoplasma and in the cell membrane.

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Product Literature References

Endothelin-1-, endothelin-A-, and endothelin-B-receptor expression is correlated with vascular endothelial growth factor expression and angiogenesis in breast cancer: P. Wulfing, et al.; Clin. Cancer Res. 10, 2393 (2004), Abstract;