Product Details
| Alternative Name: | ETA-receptor |
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| Host: | Sheep |
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| Immunogen: | Synthetic peptide corresponding to aa 410-422 (Q410EQNHNTERSSHK422) of C-terminal rat ETA-receptor (endothelin A receptor). |
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| UniProt ID: | P26684 |
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| Species reactivity: | Human, Mouse, Rat
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| Specificity: | Recognizes an epitope on the cytoplasmic side of the ETA-receptor. |
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| Applications: | IHC, WB
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| Recommended Dilutions/Conditions: | Immunohistochemistry (paraffin sections, 4µg/ml)
Western Blot (10-20µg/ml using ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | As this antibody is generated against the cytosolic portion of endothelin A receptor, permeabilization is required for immunochemical analysis.
Detects bands of 34-52kDa by Western blot.
Does not block the binding of endothelins to the receptor. |
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| Purity Detail: | Epitope-affinity purified. |
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| Formulation: | Liquid. In TRIS-citrate-phosphate, pH 7-8, containing 0.1% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Endothelins are a family of peptides that are potent vasoconstrictors produced by mammalian vascular endothelial cells. The receptors for the endothelins are found on various tissues including, but not restricted to, lung and intestine. |
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| Regulatory Status: | RUO - Research Use Only |
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Figure: Immunohistochemical staining of invasive ductal carcinoma (G2) using PAb to Endothelin A Receptor (Prod. No.
ALX-210-507A). Expression can be seen in the cytoplasma and in the cell membrane."
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Product Literature References
Changes by tacrolimus of the rat aortic proteome: Involvement of endothelin-1: J.J. Zamorano-Leon, et al.; Transpl. Immunol.
26, 191 (2012),
Application(s): Western blot,
Abstract;
Expression patterns of endothelin‐1 and its receptors in colorectal cancer: P. Liakou, et al.; J. Surg. Oncol.
105, 643 (2012),
Abstract;
Expression of endothelin receptor subtypes in bronchial tumors: S. Blouquit-Laye, et al.; Oncol. Rep.
23, 457 (2010),
Application(s): Immunohistochemistry on paraffin-embedded tissue sections,
Abstract;
Relationship between endothelin-1 and interleukin-1beta in inflamed periodontal tissues: T. Rikumaru, et al.; Biomed. Res.
30, 349 (2009),
Application(s): Western blot,
Abstract;
Selective ETAR antagonist atrasentan inhibits hypoxia-induced breast cancer cell invasion: M. Smollich, et al.; Breast Cancer Res. Treat.
108, 175 (2008),
Abstract;
Endothelin-3 growth factor levels decreased in cervical cancer compared with normal cervical epithelial cells: D.J. Sun, et al.; Hum. Pathol.
38, 1047 (2007),
Application(s): Immunohistochemistry on paraffin-embedded tissue sections,
Abstract;
Expression of the Endothelin-axis in the different histologic subtypes of renal cell carcinoma: a tissue microarray analysis: E. Hermann, et al.; Oncol. Rep.
17, 275 (2007),
Application(s): Immunohistochemistry on paraffin-embedded tissue sections,
Abstract;
Beta2-adrenoceptor agonist modulates endothelin-1 receptors in human isolated bronchi: C. Faisy, et al.; Am. J. Respir. Cell. Mol. Biol.
34, 410 (2006),
Application(s): Immunohistochemistry on paraffin-embedded tissue sections,
Abstract;
Inhibition of carotis venous bypass graft disease by intraoperative nucleic acid-based therapy in rabbits: B. Kusch, et al.; Thorac. Cardiovasc. Surg.
54, 388 (2006),
Application(s): Immunohistochemistry on paraffin-embedded tissue sections,
Abstract;
Endothelin-1-, endothelin-A-, and endothelin-B-receptor expression is correlated with vascular endothelial growth factor expression and angiogenesis in breast cancer: P. Wulfing, et al.; Clin. Cancer Res.
10, 2393 (2004),
Abstract;
Endothelin-1 synthesis and endothelin B receptor expression in human coronary artery smooth muscle cells and monocyte-derived macrophages is up-regulated by low density lipoproteins: C. Haug, et al.; J. Mol. Cell. Cardiol.
33, 1701 (2001),
Application(s): Western blot,
Abstract;
