Endothelin B receptor, pAb



ALX-210-506A-C250	250 µg	314.00 USD

Product Specification

Alternative Name:	ETB-receptor

Concentration:	1mg/ml

Purity Detail:	Epitope-affinity purified and sterile filtered.

Formulation:	Liquid. In TRIS-citrate-phosphate buffer, pH 7-8, containing 0.1% sodium azide.

Immunogen:	Synthetic peptide corresponding to aa 424-437 (K⁴²⁴ANDHGYDN FRSSNN⁴³⁷) of C-terminal rat ET_B-receptor (endothelin B receptor).

Source/Host:	From sheep.

Specificity:	Recognizes the cytoplasmic side of human, mouse, rat and sheep ET_B-receptor. Detects isoforms A and C in human. Detects bands of 34-52kDa. Does not block the binding of endothelins to the receptor.

Application:	Immunohistochemistry (paraffin sections (2.5µg/ml); frozen sections (10µg/ml)) Western Blot

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Miscellaneous/General:	Endothelins are a family of peptides that are potent vasoconstrictors produced by mammalian vascular endothelial cells. The receptors for the endothelins are found on various tissues including, but not restricted to, lung and intestine.

Background / Technical Information:	Note: This PAb was generated against the cytoplasmic portion of ET_B-receptor, permeabilization is required for immunochemical analysis.

Figure 1: Immunohistochemistry (frozen sections) using PAb to Endothelin B Receptor (Prod. No. ALX-210-506A) on rat superior mesenteric arteries. Arrow points at collagen bands, arrow head indicates ET_B-receptor expression on smooth muscle cells.

Figure 2: Immunohistochemical staining of invasive ductal carcinoma (G2) using PAb to Endothelin B Receptor (Prod. No. ALX-210-506A). Expression can be seen in the cytoplasma, in the cell membrane and in microvessels (arrow).

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Product Literature References

Endothelin-1-, endothelin-A-, and endothelin-B-receptor expression is correlated with vascular endothelial growth factor expression and angiogenesis in breast cancer: P. Wulfing, et al.; Clin. Cancer Res. 10, 2393 (2004), Abstract;