Progranulin (mouse), pAb



ALX-210-497-C100	100 µg	336.00 USD

Product Specification

Alternative Name:	Proepithelin, PC cell-derived growth factor, PEPI

Concentration:	~1mg/ml

Formulation:	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.

Clone:	PG319-1

Isotype:	Rat IgG2a

Immunogen:	Recombinant mouse progranulin expressed in HEK293 cells.

Source/Host:	From rat.

Specificity:	Recognizes mouse progranulin. Weakly cross-reacts with human progranulin.

Application:	ELISA (direct and indirect: 1:2’000-1:5’000) Western Blot (1:2’000-1:5’000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Use/Stability:	Stable for up to 6 months when stored at -20°C.

Handling:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Figure: Western blot analysis of mouse progranulin using PAb to Progranulin (mouse) (Prod. No. ALX-210-497) at dilution 1:2’000.1. Progranulin (mouse) (rec.) (Prod. No. ALX-201-389)2. Progranulin (human) (rec.) (Prod. No. ALX-201-377)3. Recombinant human Granulin C-FLAG protein4. Recombinant human Granulin F-FLAG protein5. ANGPTL3 (human) (rec.) (Prod. No. ALX-201-359) (negative control)

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