DLK1 (human), pAb



ALX-210-495-C100	100 µg	278.00 USD

Product Specification

Alternative Name:	Protein delta homolog 1 , pG2 , Fetal antigen 1, FA1

Concentration:	~1mg/ml

Purity Detail:	Purified IgG.

Formulation:	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.

Immunogen:	Recombinant human DLK1 (extracellular domain (ECD)).

Source/Host:	From rat.

Specificity:	Recognizes human DLK1.

Application:	ELISA (direct and indirect: 1:2’000-1:10’000) Flow Cytometry (1:100-1:500) Western Blot (1:2’000-1:10’000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Use/Stability:	Stable for up to 6 months after receipt when stored at -20°C.

Handling:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Figure 1: Western blot analysis of human Dlk1 using Dlk1 (human), pAb (Prod. No. ALX-210-495) at 1:2’000 dilution.
A.
1. Dlk1 (human):Fc (human) (rec.) (Prod. No. ALX-201-427)
2. Human full length Dlk1 transfected cell lysate (HEK 293)
3. Mock transfected HEK 293 cell lysate

B.
1. Dlk1 (human):Fc (human) (rec.) (Prod. No. ALX-201-427)
2. Dlk1 (mouse):Fc (human) (rec.) (Prod. No. ALX-201-416)
3. DNER (Extracellular Domain) (human):Fc (human) (rec.) (Prod. No. ALX-201-428)
4. DLL1 (human):Fc (human) (rec.) (Prod. No. ALX-201-425)
5. DLL3 (human):Fc (human) (rec.) (Prod. No. ALX-201-422)
6. DLL4 (human):Fc (human) (rec.) (Prod. No. ALX-201-386)
7. Jagged-1 (human):Fc (human) (rec.) (Prod. No. ALX-201-390)
8. FGF-23 (human):Fc (human) (rec.) (Prod. No. ALX-201-418)

Figure 2: Flow Cytometry. DLK1 (full length) -transfected HEK 293 cells were stained significantly by PAb to Dlk1 (human) (Prod. No. ALX-210-495).

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