ANGPTL4 (79-101) (human), pAb



ALX-210-458-C100	100 µg	278.00 USD

Product Specification

Alternative Name:	Fasting-induced adipose factor, FIAF, HFARP, Angiopoietin-like protein 4, Hepatic fibrinogen/angiopoietin-related protein, PGAR

Concentration:	1mg/ml.

Purity Detail:	Purified IgG.

Formulation:	Liquid. In PBS, pH 7.4. Contains no preservatives.

Immunogen:	Synthetic peptide corresponding to aa 79-101 (A⁷⁹CQGTEGSTDLPLAPESRVDPEV¹⁰¹) of human ANGPTL4 (angiopoietin-like protein 4).

Source/Host:	From rabbit.

Specificity:	Recognizes the coiled-coil domain of human ANGPTL4. Weakly cross-reacts with human ANGPTL6. Does not cross-react with other ANGPTL family proteins.

Application:	ELISA (direct and indirect: 1:2’000-1:5’000) Western Blot (1:2’000-1:5’000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Handling:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Crossreactivity Proteins:	ANGPTL6 (weak)

Figure: Western blot analysis using ANGPTL4 (79-101) (human), pAb (Prod. No. ALX-210-458) at 1:2’000 dilution.
1. FLAG^®-tagged ANGPTL1 (human) (rec.)
2. FLAG^®-tagged ANGPTL2 (human) (rec.)
3. FLAG^®-tagged ANGPTL3 (human) (rec.) (Prod. No. ALX-201-359)
4. FLAG^®-tagged ANGPTL4 (Fibrinogen-like Domain) (human) (rec.) (Prod. No. ALX-201-379)
5. FLAG^®-tagged ANGPTL4 (Coiled-coil Domain) (human) (rec.) (Prod. No. ALX-201-373)
6. FLAG^®-tagged ANGPTL6 (human) (rec.) (Prod. No. ALX-201-337)
7. FLAG^®-tagged ANGPTL7 (human) (rec.) (Prod. No. ALX-201-368)
8. FLAG^®-tagged RBP4 (mouse) (rec.) (negative control) (Prod. No. ALX-201-353)

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