Omentin 1 (human), pAb



ALX-210-454-C100	100 µg	292.00 USD

Product Specification

Alternative Name:	Intelectin-1, Intestinal lactoferrin receptor, Galactofuranose-binding lectin, Endothelial lectin HL-1

Concentration:	1mg/ml.

Purity Detail:	Purified IgG.

Formulation:	Liquid. In PBS, pH 7.4. Contains no preservatives.

Immunogen:	Synthetic peptide corresponding to aa 95-115 (T⁹⁵VGDRWSSQQGSKADYPEGDG¹¹⁵) of human omentin 1 (intelectin-1).

Source/Host:	From rabbit.

Specificity:	Recognizes human omentin 1.

Application:	ELISA (direct and indirect: 1:2'000-1:5'000) Western Blot (1:2'000-1:5'000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Handling:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Miscellaneous/General:	Omentin 1 (also named intelectin-1, endothelial lectin HL-1 and intestinal lactoferrin receptor) has been identified as a visceral fat (or omental fat) depot-specific adipokine. Omentin 1 is a secreted protein of ~38 kDa containing a fibrinogen-related domain. Omentin 1 is highly expressed in the omental fat, and much less in intestine, lung and heart. Addition of recombinant omentin in vitro did not affect basal, but enhanced insulin-stimulated glucose uptake in subcutaneous as well as in omental human adipocytes. Omentin 1 triggers AKT signalling in the absence of insulin.

Figure 1: Western blot analysis using PAb to Omentin 1 (human) (Prod. No. ALX-210-454) at 1:2’000 dilution. 1. His-tagged omentin 1 (human) (rec.). 2. His-tagged visfatin (human) (rec.) (negative control). 3. His-tagged human clusterin (human) (rec.) (negative control).

Figure 2: Indirect ELISA of recombinant human omentin 1 and peptide using PAb to Omentin 1 (human) (Prod. No. ALX-210-454) at 1:50’000 dilution. *Other Peptide: Synthetic human visfatin.

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