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GITRL (mouse) polyclonal antibody

ALX-210-375-C100 100 µg 280.00 USD
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Product Specification

Alternative Name:AITRL, TNFSF 18, Glucocorticoid-induced TNF receptor ligand, Activation-inducible TNF-related ligand
Immunogen:Recombinant mouse GITRL.
UniProt ID:Q7TS55
Species reactivity:Mouse
Specificity:Recognizes both natural and recombinant GITRL.
Crossreactivity:Does not cross-react with human GITRL (AITRL).
Applications:ELISA, Flow Cytometry, WB
Recommended Dilutions/Conditions:ELISA (direct and indirect: 1:5,000-1:10,000)
Flow Cytometry (1:1,000)

Western Blot (1:2,000-1:5,000 using ECL; suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5 % skim milk; suggested incubation time is 1 hour at room temperature).
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Formulation:Liquid. In PBS, pH 7.4. Contains no preservatives.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Shipping:Shipped on Blue Ice
Short Term Storage:+4°C
Long Term Storage:-20°C
Figure: left) Flow cytometry analysis of Con A-stimulated mouse splenic  T cells with anti-GITRL PAb (Prod. No. ALX-210-375). right) Flow cytometry analysis of HEK293 cells overexpressing GITRL with anti-GITRL PAb (Prod. No. ALX-210-375).

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Product Literature References

Soluble glucocorticoid-induced tumor necrosis factor receptor (sGITR) stimulates osteoclast differentiation in response to receptor activator of NF-kappaB ligand (RANKL) in osteoclast cells: H.H. Shin, et al.; Bone 36, 832 (2005), Abstract;
The soluble glucocorticoid-induced tumor necrosis factor receptor causes cell cycle arrest and apoptosis in murine macrophages: H.H. Shin, et al.; BBRC 316, 24 (2004), Abstract;
Identification of a ligand for glucocorticoid-induced tumor necrosis factor receptor constitutively expressed in dendritic cells: K.Y. Yu, et al.; BBRC 310, 433 (2003), Abstract;

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