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Toll-like receptor 3 (mouse) polyclonal antibody

 
ALX-210-367-C100 100 µg 370.00 USD
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Product Specification

Alternative Name:TLR3, CD283
 
Host:Rabbit
 
Immunogen:Synthetic peptides corresponding to aa 135-150, 828-844 and 876-891 of mouse TLR3 (Toll-like receptor 3).
 
UniProt ID:Q99MB1
 
Species reactivity:Mouse
 
Crossreactivity:Does not cross-react with human TLR3.
 
Applications:Flow Cytometry, IHC (FS), IHC (PS), WB
 
Recommended Dilutions/Conditions:Flow Cytometry (cell surface & intracellular, 1µg/106 cells)
Immunohistochemistry (paraffin sections, 1:100; frozen sections, 1:20-1:1,000)
Western Blot (1-3µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Positive Control:Mouse spleen cell whole cell lysate can be used as positive control.
 
Purity Detail:Protein G-affinity purified.
 
Formulation:Liquid. In PBS containing 0.05% sodium azide.
 
Use/Stability:Stable for 6 months when stored at +4°C.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Technical Info/Product Notes:Replacement for ADI-CSA-790
 
ALX-210-367 FC
Intracellular flow analysis of TLR3 in Balb/c mouse splenocytes using PAb to Toll-like Receptor 3 (mouse) (Prod. No. ALX-210-367) at 1µg/106 cells. Shaded histogram represents cells without antibody; green represents rabbit IgG isotype control; red represents PAb to Toll-like Receptor 3 (mouse).
ALX-210-367 WB
Western blot analysis of TLR3 in 15µg/lane mouse spleen tissue lysate using PAb to Toll-like Receptor 3 (mouse) (Prod. No. ALX-210-367).
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ALX-210-367 FC ALX-210-367 WB

Product Literature References

Expression and regulation of Toll-like receptors in lupus-like immune complex glomerulonephritis of MRL-Fas(lpr) mice: P.S. Patole, et al.; Nephrol. Dial. Transplant. 21, 3062 (2006), Abstract;
Toll-like receptor-7 modulates immune complex glomerulonephritis: R.D. Pawar, et al.; J. Am. Soc. Nephrol. 17, 141 (2006), Abstract;
Viral double-stranded RNA aggravates lupus nephritis through Toll-like receptor 3 on glomerular mesangial cells and antigen-presenting cells: P.S. Patole, et al.; J. Am. Soc. Nephrol. 16, 1326 (2005), Abstract;

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