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PTX3 polyclonal antibody

ALX-210-365-C050 50 µg 398.00 USD
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Product Specification

Alternative Name:Pentraxin 3, TSG-14, TNF stimulated gene-14
Immunogen:Recombinant human PTX3.
UniProt ID:P26022
Species reactivity:Human, Mouse, Rat
Specificity:Recognizes human, mouse and rat PTX3 and chicken homolog PPTX.
Crossreactivity:Weakly cross-reacts with bovine PTX3.
Applications:ELISA, ICC, IHC (FS), IHC (PS), IP, WB
Recommended Dilutions/Conditions:ELISA (detection, 1:20,000)
Western Blot (500ng/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Excellent for use as detection antibody in an ELISA format together with mAb to PTX3 (Prod. No. ALX-804-464 (capture)) and PTX3 standard (Prod. No. ALX-201-124).
Purity Detail:Affinity purified.
Formulation:Liquid. In PBS containing 0.1% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Figure:Lysates of normal lung fibroblasts (WI38) and of human fibrosarcoma (8387) were probed with the PTX3, pAb, (Prod. No. ALX-210-365), over night at +4 °C at a concentration of 500ng/ml in 5% dry milk in PBS+/+ containing 0.05% Tween 20. The signal was revealed with an HRP-conjugated anti-rat IgG secondary antibody in 5% dry milk PBS+/+ containing 0.05% Tween 20 after incubation for 1 hour at room temperature and IMMOBILON Western Chemiluminescent substrate from Millipore.
210-365 2
Figure 2: Pulmonary PTX3 expression by immunohistochemistry (IHC) using PTX3, pAb (Prod. No. ALX-210-365).
Method: Photomicrograph of PTX3 stained lung tissue of Cigarette Smoke (CS)-exposed WT mice. Mice were exposed to CS for 4 weeks (subacute).
(V: vein, A: artery, AW: airway. Magnification 200×). In courtesy of Guy Brusselle.
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Product Literature References

Early expression of PTX3 in Aspergillus fumigatus infected rat cornea: J. Zhang, et al.; Int. J. Ophthalmol. 11, 1084 (2018), Abstract; Full Text
Cigarette smoke induces PTX3 expression in pulmonary veins of mice in an IL-1 dependent manner: N.S. Pauwels, et al.; Respir. Res. 11, 134 (2010), Abstract;
Pentraxin 3 is elevated in haemodialysis patients and is associated with cardiovascular disease: M. Boehme, et al.; Nephrol. Dial. Transplant. 22, 2224 (2007), Abstract;
Elevated maternal levels of the long pentraxin 3 (PTX3) in preeclampsia and intrauterine growth restriction: I. Cetin, et al.; Am. J. Obstet. Gynecol. 194, 1374 (2006), Abstract; Full Text
A secretory-type protein, containing a pentraxin domain, interacts with an A-type K+ channel: D.E. Duzhyy, et al.; J. Biol. Chem. 280, 15165 (2005), Abstract;
The long pentraxin Ptx3 is synthesized in IgA glomerulonephritis and activates mesangial cells: B. Bussolati, et al.; J. Immunol. 170, 1466 (2003), Abstract;
PTX3, A prototypical long pentraxin, is an early indicator of acute myocardial infarction in humans: G. Peri, et al.; Circulation 102, 636 (2000), Abstract; Full Text
Expression of a long pentraxin, PTX3, by monocytes exposed to the mycobacterial cell wall component lipoarabinomannan: V. Vouret-Craviari; Infect. Immun. 65, 1345 (1997), Abstract;
Inducible expression of PTX3, a new member of the pentraxin family, in human mononuclear phagocytes: V.V. Alles, et al.; Blood 84, 3483 (1994), Abstract; Full Text

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