MDA5 (mouse), pAb (AL180)



ALX-210-352-R100	100 µl	409.00 USD

Product Specification

Alternative Name:	Helicard, Melanoma differentiation-associated gene 5

Formulation:	Liquid. Contains 0.02% sodium azide.

Immunogen:	Recombinant mouse MDA5 (melanoma differentiation-associated gene 5) (aa 2-208).

Source/Host:	From rabbit.

Specificity:	Recognizes mouse MDA5.

Application:	Western Blot

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Use/Stability:	Stable for at least 1 year after receipt when stored as recommended.

Handling:	Avoid freeze/thaw cycles.

Miscellaneous/General:	MDA5 is a highly conserved helicase whose ATPase activity can be stimulated by RNA and less potently by DNA. It contains two N-terminal CARD domains and a C-terminal helicase domain. The helicase domain, once freed from the CARD domains, then translocates from the cytoplasm to the nucleus where it acts on chromatin architecture. This could allow a more easy access of CAD, thereby accelerating the degradation of genomic DNA.

Figure: Detection of cleavage of endogenous mouse MDA5 by Western blot.Method: Murine A20 B lymphoma cells were incubated either with medium (1) or with 200ng/ml of rhsFasL (2) in the presence of 1µg/ml Enhancer for Ligands (rhsFasL Set, Prod. No. ALX-850-014) for 4 hours. Cells were then harvested, lysed and the cell extracts were resolved by SDS-PAGE under reducing conditions. After transfer to nitrocellulose the membrane was incubated with the PAb to MDA5 (mouse) (Prod. No. ALX-210-352) at a dilution of 1:1’000 for 2 hours. Detection of MDA5 was performed using a peroxidase-conjugated antibody to rabbit Ig (Jackson Lab.) followed by a chemiluminescence detection system.Note: In non-stimulated A20S cells (1) MDA5 has a molecular weight of 140kDa. Upon FasL-induced activation (2), cleavage products of 120, 41/45, 30 and 28kDa can be detected.

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Product Literature References

Overexpression of Helicard, a CARD-Containing Helicase Cleaved during Apoptosis, Accelerates DNA Degradation: M. Kovacsovics, et al.; Curr. Biol. 12, 838 (2002), Abstract;

General Literature References

Mda-5: An interferon-inducible putative RNA helicase with double-stranded RNA-dependent ATPase activity and melanoma growth-suppressive properties: D.C. Kang, et al.; PNAS 99, 637 (2002), Abstract;