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Osteocalcin (mouse) (NT) polyclonal antibody

 
ALX-210-333-C100 100 µg 722.00 USD
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Product Specification

Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to aa 1-20 (Y1LGASVPSPDP
LEPTREQCE20) of intracellular N-terminal mouse osteocalcin.
 
UniProt ID:P86547
 
Species reactivity:Mouse
 
Specificity:Recognizes intracellular N-terminal osteocalcin.
 
Crossreactivity:Does not cross-react with rat osteocalcin.
 
Applications:IHC (PS)
 
Recommended Dilutions/Conditions:Immunohistochemistry (paraffin sections, 1-2µg/ml, after trypsin treatment)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Not suitable for WB.
 
Purity Detail:Protein A-affinity and epitope-affinity purified.
 
Formulation:Lyophilized from 10mM PBS, pH 7.4, containing 1% BSA.
 
Reconstitution:Reconstitute with 50µl of Reconstitution Solution (water containing 0.1% sodium azide) to a final concentration of 2mg/ml. Further dilutions should be made using 10mM PBS, pH 7.4, containing 1% BSA and 0.1% sodium azide.
 
Use/Stability:Reconstituted solution can be stored at 4°C for 6 months and at -20°C for 1 year.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 

Product Literature References

Insulin-Like Growth Factor I Does Not Drive New Bone Formation in Experimental Arthritis: M.N. van Tok, et al.; PLoS One 11, e0163632 (2016), Application(s): Immunohistochemistry, ankle and tow section osteoblasts, Abstract; Full Text

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