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PARP-1 polyclonal antibody

ALX-210-302-R100 100 µl 296.00 USD
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Product Specification

Alternative Name:Poly(ADP-ribose) polymerase-1
Immunogen:Recombinant human PARP-1 (poly(ADP-ribose) polymerase-1) (aa 1-1014).
UniProt ID:P09874
Species reactivity:Human, Mouse
Bovine, Monkey
Crossreactivity:Does not cross-react with PARP-2.
Applications:ELISA, ICC, IHC (FS), IHC (PS), IP, WB
Recommended Dilutions/Conditions:Immunocytochemistry (1:4,000)
Immunoprecipitation (1:400)
Western Blot (1:4,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects bands of ~116kDa (PARP-1) and ~85kDa (apoptosis-induced cleavage fragment) by Western blot.
Formulation:Liquid. Neat serum containing 0.1% sodium azide.
Use/Stability:Stable for at least one year when stored at +4°C.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:+4°C
ALX-210-302 WB
Figure: Western blot using rabbit polyclonal anti PARP-1.
Lane 1: recombinant human PARP-1 (ALX-201-053, 5 ng).
Lane 2: Total HeLa cell extract.
Lane 3: Total MEF PARP1+/+ cell extract.
Lane 4: Total MEF PARP1-/- cell extract.
Lane 5: Lysate (50 μg) from HeLa cells.
Lane 6: Lysate (50 μg) from HeLa PARP-1sh cells.
Lane 7: Lysate (50 μg) from HeLa cells treated for 8 hours with Doxorubicin 5 μg/ml.
Lane 8: Lysate (50 μg) from HEK293 cells.
Lane 9: Lysate (50 μg) from HEK293 cells.
Lane 10: Lysate (50 μg) from HEK293 cells transfected with PARP-1 siRNA.
Lane 11: Lysate (50 μg) from HEK293 cells transfected with control siRNA.
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ALX-210-302 WB

Product Literature References

Posttranscriptional Regulation of HIV-1 Gene Expression during Replication and Reactivation from Latency by Nuclear Matrix Protein MATR3: A. Sarracino, et al.; Mbio 9, e02158-18 (2018), Abstract; Full Text
PARP1 promotes gene expression at the post-transcriptiona level by modulating the RNA-binding protein HuR: Y. Ke, et al.; Nat. Commun. 8, 14632 (2017), Abstract; Full Text
A PARP1-ERK2 synergism is required for the induction of LTP: L. Visochek, et al.; Sci. Rep. 6, 24950 (2016), Application(s): Western blot, Abstract; Full Text
Lysosomal calcium signalling regulates autophagy through calcineurin and ​TFEB: D.L. Medina, et al.; Nat. Cell. Biol. 17, 288 (2015), Application(s): Western Blotting, Abstract;
NF-κB transcriptional activation by TNFα requires phospholipase C, extracellular signal-regulated kinase 2 and poly(ADP-ribose) polymerase-1: B. Vuong, et al.; J. Neuroninflammation 12, 229 (2015), Application(s): Western blot detecting PARP-1 expression, Abstract; Full Text
The prevention of spontaneous apoptosis of follicular lymphoma B cells by a follicular dendritic cell line: involvement of caspase-3, caspase-8 and c-FLIP: J.J. Goval, et al.; Haematologica 93, 1169 (2008), Application(s): WB using human follicular lymphoma lysate, Abstract; Full Text

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