Cyclophilin B, pAb

[CyPB]



ALX-210-237/1-C100	100 µg	531.00 USD

Product Specification

Concentration:	1mg/ml.

Purity Detail:	Epitope-affinity purified IgG.

Formulation:	Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.

Immunogen:	Synthetic peptide corresponding to aa 194-208 (C¹⁹⁴GKIEVEKPFAIAKE²⁰⁸) of human cyclophilin B.

Source/Host:	From rabbit.

Specificity:	Recognizes human, mouse, rat and dog cyclophilin B. Detects a band of ~19kDa by Western blot.

Application:	Western Blot (0.5µg/ml)

Shipping:	SHIPPED ON BLUE ICE

Long Term Storage:	-20°C

Handling:	Avoid freeze/thaw cycles.

Miscellaneous/General:	Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents - cyclosporin A (CsA) (see Prod. No. ALX-380-002) or FK506 (see Prod. No. ALX-380-008). Proteins which bind FK506 are termed FK506 Binding Proteins (FKBP's) and those which bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation. Thus, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins.

General Literature References

Transport activity and surface expression of the Na+-Ca2+ exchanger NCX1 are inhibited by the immunosuppressive agent cyclosporin A and by the nonimmunosuppressive agent PSC833 C. Kimchi-Sarfaty, et al. J. Biol. Chem. 277 2505 (2002) Abstract Full Text