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VE-cadherin polyclonal antibody

ALX-210-232-C100 100 µg Purified Inquire for pricing
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Product Specification

Alternative Name:Vascular endothelial cadherin, Cadherin-5, 7B4 antigen, CD144
Immunogen:Recombinant human VE-cadherin (aa 1-258).
UniProt ID:P33151
Species reactivity:Human, Mouse, Rat
Applications:ELISA, Flow Cytometry, ICC, IHC (FS), WB, FUNC
Recommended Dilutions/Conditions:Immunohistochemistry (formalin or acetone-fixed frozen sections, 1µg/ml)
Western Blot (see protocol)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Functional Application: Interferes with VE-cadherin adhesion (reversible endothelium disruption). This effect mimics the transmigration of leukocytes or cancer cells through endothelium.
Not recommended for IHC (PS).
Purity Detail:Affinity purified using recombinant VE-cadherin fragments.
Formulation:Liquid. In PBS, pH 7.2-7.4.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Short Term Storage:+4°C
Long Term Storage:-20°C

Product Literature References

c-Met-mediated endothelial plasticity drives aberrant vascularization and chemoresistance in glioblastoma: M. Huang, et al.; J. Clin. Invest. 126, 1801 (2016), Application(s): Immunoblotting, Abstract;
VE-cadherin-p120 interaction is required for maintenance of endothelial barrier function: S. Iyer, et al.; Am. J. Physiol. Lung Cell Mol. Physiol. 286, L1143 (2004), Abstract;
Alteration of endothelial cell monolayer integrity triggers resynthesis of vascular endothelium cadherin: D. Gulino, et al.; J. Biol. Chem. 273, 29786 (1998), Abstract; Full Text

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VE-cadherin polyclonal antibody (biotin conjugate) 

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