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PARP-1 (215-228) polyclonal antibody

ALX-210-220-R100 100 µl 296.00 USD
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Product Specification

Alternative Name:Poly(ADP-ribose) polymerase-1
Immunogen:Synthetic peptide corresponding to aa 215-228 (G215VDEVAKKKSKKEK228) of human PARP-1 (poly(ADP-ribose) polymerase-1).
UniProt ID:P09874
Species reactivity:Human
Bovine, Chicken
Applications:ELISA, ICC, WB
Recommended Dilutions/Conditions:ELISA (1:210,000)
Immunocytochemistry (1:50)
Western Blot (1:2,000-1:5,000)
Optimal conditions must be determined individually for each application.
Application Notes:Detects bands of ~116kDa (full-length PARP-1) and ~85kDa (apoptosis-induced cleavage fragment) by Western blot.
Formulation:Liquid. Contains 0.02% sodium azide.
Shipping:Shipped on Blue Ice
Long Term Storage:+4°C
Scientific Background:During apoptosis PARP-1 is cleaved between Asp214 and Gly215 to yield two fragments of 29kDa and 85kDa. This cleavage site is conserved in human, bovine and chicken PARP-1.

Product Literature References

Characterization of stress response in human retinal epithelial cells: V. Giansanti, et al.; J. Cell. Mol. Med. 17, 103 (2013), Application(s): WB using human adult retinal pigmented epithelial (ARPE-19) cell lysate, Abstract; Full Text
Characterization of antibodies specific for the caspase cleavage site on poly(ADP-ribose) polymerase: specific detection of apoptotic fragments and mapping of the necrotic fragments of poly(ADP-ribose) polymerase: F.R. Sallmann, et al.; Biochem. Cell Biol. 75, 451 (1997), Abstract;
Structural and functional analysis of poly(ADP ribose) polymerase: an immunological study: D. Lamarre, et al.; Biochim. Biophys. Acta 950, 147 (1988), Abstract;

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The PARP Family & ADP Ribosylation
The PARP Family & ADP Ribosylation
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