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PARP-1 (197-214) polyclonal antibody

ALX-210-219-R100 100 µl 215.00 USD
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Product Specification

Alternative Name:Poly(ADP-ribose) polymerase-1
Immunogen:Synthetic peptide corresponding to aa 197-214 (K197QLPGVKSE
GKRKGDEVD214) of human PARP-1 (poly(ADP-ribose) polymerase-1).
UniProt ID:P09874
Species reactivity:Human
Bovine, Chicken
Applications:ELISA, ICC, IP, WB
Recommended Dilutions/Conditions:ELISA (1:100,000)
Immunocytochemistry (1:50)
Western Blot (1:10,000-1:15,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects bands of ~116kDa (full length PARP-1) and ~29kDa (apoptosis-induced cleavage fragment) by Western blot.
Formulation:Liquid. Contains 0.02% sodium azide.
Shipping:Shipped on Blue Ice
Long Term Storage:-80°C
Scientific Background:During apoptosis PARP-1 is cleaved between Asp214 and Gly215 to yield two fragments of 29kDa and 85kDa. This cleavage site is conserved in human, bovine and chicken PARP-1.

Product Literature References

Autophagy Deficiency Diminishes Indomethacin-Induced Intestinal Epithelial Cell Damage through the Activation of ERK/Nrf2/HO-1 Pathway: S. Harada, et al.; J. Pharmacol. Exp. Ther. 355, 353 (2015), Application(s): Western Blot, Abstract; Full Text
Structural and functional analysis of poly(ADP ribose) polymerase: an immunological study: D. Lamarre, et al.; Biochim. Biophys. Acta 950, 147 (1988), Abstract;

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