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Estrogen receptor α polyclonal antibody

ALX-210-201-C050 50 µg 616.00 USD
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Product Specification

Alternative Name:ERα
Immunogen:Synthetic peptide corresponding to aa 1-21 (M1TMTLHTKASGMALLHQIQGN21) of N-terminal human ERα (estrogen receptor α). This sequence is completely conserved in rat and mouse ERα and is highly conserved in sheep, pig, dog, bovine and chicken ERα.
UniProt ID:P03372
Species reactivity:Human, Rat
Specificity:Recognizes endogenous ERα.
Crossreactivity:Does not cross-react with ERβ.
Applications:IHC (FS), WB
Recommended Dilutions/Conditions:Immunohistochemistry (frozen sections, 1-5µg/ml)
Western Blot (1µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~64kDa by Western blot.
Purity Detail:Epitope-affinity purified IgG.
Formulation:Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Figure: Immunohistochemistry of ERa in rat prostate using PAb to Estrogen Receptor a (Prod. No. ALX-210-201).
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Product Literature References

Cytochromes 1A1/1B1- and catechol-O-methyltransferase-derived metabolites mediate estradiol-induced antimitogenesis in human cardiac fibroblast: R.K. Dubey, et al.; J. Clin. Endocrinol. Metab. 90, 247 (2005), Abstract; Full Text
Differential Regulation of Estrogen Receptor Subtypes alpha and beta in Human Aortic Smooth Muscle Cells by Oligonucleotides and Estradiol: F. Barchiesi, et al.; J. Clin. Endocrinol. Metab. 89, 2373 (2004), Abstract;

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