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Green fluorescent protein polyclonal antibody

 
ALX-210-199-R100 100 µl 378.00 USD
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Replaces Prod. #: ALX-210-199/1

Product Specification

Alternative Name:GFP
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to aa 26-39 (H26KFSVSGEGEGDAT39) of the jellyfish Aequorea victoria GFP (green fluorescent protein). This sequence is completely conserved between GFP and the modified and enhanced Blue, Green and Yellow FP variants.
 
UniProt ID:P42212
 
Species reactivity:Species independent
 
Specificity:Recognizes GFP and GFP fusion proteins.
 
Applications:ICC, WB
 
Recommended Dilutions/Conditions:Immunocytochemistry (1:200)
Western Blot (0.5µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~28 kDa by Western blot.
 
Purity Detail:Epitope-affinity purified.
 
Formulation:Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Green Fluorescent Protein (GFP) is responsible for bioluminescence in the jellyfish Aequorea victoria. This protein has quickly become a powerful research tool for assessing gene expression and subcellular protein distribution in fixed or living cells. GFP is excited by and brightly fluoresces when exposed to UV or blue light. This feature makes it ideal as a marker for use in fluorescence microscopy, cytometry, tagging fusion proteins, and assaying transcriptional regulation from gene promoters in vivo. Numerous GFP variants with enhanced and shifted emission spectra (blue, green, and yellow) have been developed through aa substitutions at specific residues.
 

Product Literature References

Massively parallel delivery of large cargo into mammalian cells with light pulses: Y.C. Wu, et al.; Nat. Methods 12, 439 (2015), Application(s): Western Blot, Abstract;

General Literature References

Understanding, improving and using green fluorescent proteins: A.B. Cubitt, et al.; TIBS 20, 448 (1995), (Review), Abstract;

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