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Glucocorticoid receptor β (human) polyclonal antibody

 
ALX-210-154-R050 50 µl 399.00 USD
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Product Specification

Alternative Name:GRβ
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to aa 728-742 (N728VMWLKPESTSHTLI742) of human GRβ (glucocorticoid receptor β).
 
UniProt ID:P04150
 
Species reactivity:Human
 
Applications:ICC, IP, WB
 
Recommended Dilutions/Conditions:Immunocytochemistry (1:1,500)
Western Blot (1:500)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Immunocytochemical staining of GRβ in HeLa cells with Prod. No. ALX-210-154 results in nuclear staining.
Detects a band of ~90kDa by Western blot.
 
Formulation:Liquid. In PBS containing 0.05% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism, and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocorticoid receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain GR.

The human GR exists in two forms, α and β, which are thought to be the result of alternative splicing of a single gene. Sequence analysis indicates that the α and β forms of human GR are 777 and 742 amino acids long, respectively. They are identical up to residue 727, after which they diverge. After ligand binding, the 94kDa GRα isoform has been observed to translocate from the cytoplasm to the nucleus where it regulates gene expression. In contrast, the 90kDa GRβ isoform does not appear to bind either glucocorticoid agonists or antagonists, and has been localized predominantly in the nucleus independent of hormone treatment in some human cell lines. Studies suggest that human GRβ might function as a dominant negative inhibitor of GRα activity.

 
210-154
Figure 1 shows a Western blot of GRb on COS-74 whole cell extract using Prod. No. ALX-210-154.
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210-154

Product Literature References

Expression and subcellular distribution of the beta-isoform of the human glucocorticoid receptor: R.H. Oakley, et al.; Endocrinology 138, 5028 (1997), Abstract; Full Text

General Literature References

The human glucocorticoid receptor beta isoform. Expression, biochemical properties, and putative function: R.H. Oakley, et al.; J. Biol. Chem. 271, 9550 (1996), Abstract; Full Text
Primary structure and expression of a functional human glucocorticoid receptor cDNA: S.M. Hollenberg, et al.; Nature 318, 635 (1985), Abstract;

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