| ALX-209-259-T100 | 100 tests | 215.00 USD | ![]() |
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| Bulk Request | |||||
| Purity Detail: | Affinity purified. |
| MW: | ~52kDa (SDS-PAGE). |
| Quantity: | 100 tests/0.5ml |
| Purity: | ≥90% |
| Formulation: | Liquid. In PBS, pH 7.4, containing 0.02% sodium azide. |
| Specificity: | Binds to phosphatidylserine (PS). |
| Application: | Detection of apoptotic cells by flow cytometry or immunofluorescence. |
| Long Term Storage: | +4°C |
| Use/Stability: | Stable for at least one year after receipt when stored at +4°C. |
| Handling: | Do not freeze. Protect from light. |
| Background / Technical Information: | UniProt ID P08758: Annexin V (human) AfCS Signaling Gateway ID A000281: Annexin V (mouse) |
| Protocol: | Flow Cytometry Protocol: - Wash cells in PBS, and resuspend in binding buffer (10mM HEPES/NaOH, pH 7.4, 140mM NaCl, 2.5mM CaCl2; filtered through 0.2µm filter). - Adjust cells to a density between 200’000 and 500’000 cells/ml. - Take 195µl cell suspension and add 5µl Annexin-ATTO 647N. Mix and incubate 10 min. in the dark. - Wash cells 1x and resuspend in 190µl binding buffer. - Add 10µl of 20µg/ml propidium iodide (PI) stock solution to stain for necrotic cells. - Analyze by FACS. Note: These are general recommendations. Optimal conditions must be determined individually for each application. |

| 20-Sep-10 | |
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