Annexin V (human), (recombinant) (ATTO 647N conjugate)



ALX-209-259-T100	100 tests	215.00 USD
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ATTO-fluorescent dyes show increased photostability, outstanding brightness and intense signals. ATTO dyes are thermally stable, resistant to environmental changes and show no significant isomerization. ATTO 647N shows red fluorescence (λabs (max): 645nm, λem (max): 669nm, εmax: 120’000).

Product Specification

Purity Detail:	Affinity purified.

MW:	~52kDa (SDS-PAGE).

Quantity:	100 tests/0.5ml

Purity:	≥90%

Formulation:	Liquid. In PBS, pH 7.4, containing 0.02% sodium azide.

Specificity:	Binds to phosphatidylserine (PS).

Application:	Detection of apoptotic cells by flow cytometry or immunofluorescence.

Long Term Storage:	+4°C

Use/Stability:	Stable for at least one year after receipt when stored at +4°C.

Handling:	Do not freeze. Protect from light.

Background / Technical Information:	UniProt ID P08758: Annexin V (human) AfCS Signaling Gateway ID A000281: Annexin V (mouse)

Protocol:	Flow Cytometry Protocol: - Wash cells in PBS, and resuspend in binding buffer (10mM HEPES/NaOH, pH 7.4, 140mM NaCl, 2.5mM CaCl₂; filtered through 0.2µm filter). - Adjust cells to a density between 200’000 and 500’000 cells/ml. - Take 195µl cell suspension and add 5µl Annexin-ATTO 647N. Mix and incubate 10 min. in the dark. - Wash cells 1x and resuspend in 190µl binding buffer. - Add 10µl of 20µg/ml propidium iodide (PI) stock solution to stain for necrotic cells. - Analyze by FACS. Note: These are general recommendations. Optimal conditions must be determined individually for each application.