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Annexin V (human), (recombinant) (ATTO 488 conjugate)

 
ALX-209-258-T100 100 tests 240.00 USD
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Product Specification

MW:~52kDa (SDS-PAGE).
 
Source:Produced in E. coli.
 
UniProt ID:P08758
 
Quantity:100 tests/0.5ml
 
Formulation:Liquid. In PBS, pH 7.4, containing 0.02% sodium azide.
 
Purity:≥90%
 
Purity Detail:Affinity purified.
 
Specificity:Binds to phosphatidylserine (PS).
 
Applications:Flow Cytometry, IF
 
Application Notes:Detection of apoptotic cells by flow cytometry or immunofluorescence.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:+4°C
 
Use/Stability:Stable for at least one year after receipt when stored at +4°C.
 
Handling:Protect from light. Do not freeze.
 
Technical Info/Product Notes:ATTO-fluorescent dyes show increased photostability, outstanding brightness and intense signals. ATTO dyes are thermally stable, resistant to environmental changes and show no significant isomerization. ATTO 488 shows bright green fluorescence (λabs (max): 501nm, λem (max): 523nm, εmax: 90'000).
 
Protocol:Flow Cytometry Protocol:
- Wash cells in PBS, and resuspend in binding buffer (10mM HEPES/NaOH, pH 7.4, 140mM NaCl, 2.5mM CaCl2; filtered through 0.2µm filter).
- Adjust cells to a density between 200’000 and 500’000 cells/ml.
- Take 195µl cell suspension and add 5µl Annexin-ATTO 488. Mix and incubate 10 min. in the dark.
- Wash cells 1x and resuspend in 190µl binding buffer.
- Add 10µl of 20µg/ml propidium iodide (PI) stock solution to stain for necrotic cells.
- Analyze by FACS.
Note: These are general recommendations. Optimal conditions must be determined individually for each application.
 

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