Annexin V (human), (recombinant) (ATTO 488 conjugate)
| ALX-209-258-T100 | 100 tests | 270.00 USD |  |
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| Bulk Request | ||||
New ATTO-fluorescent dyes show increased photostability, outstanding brightness and intense signals. ATTO dyes are thermally stable, resistant to environmental changes and show no significant isomerization. ATTO 488 shows bright green fluorescence (λabs (max): 501nm, λem (max): 523nm, εmax: 90'000).
Product Specification
| Purity Detail: | Affinity purified. |
| MW: | ~52kDa (SDS-PAGE). |
| Quantity: | 100 tests/0.5ml |
| Purity: | ≥90% |
| Formulation: | Liquid. In PBS, pH 7.4, containing 0.02% sodium azide. |
| Specificity: | Binds to phosphatidylserine (PS). |
| Application: | Detection of apoptotic cells by flow cytometry or immunofluorescence. |
| Shipping: | SHIPPED ON BLUE ICE |
| Long Term Storage: | +4°C |
| Use/Stability: | Stable for at least one year after receipt when stored at +4°C. |
| Handling: | Do not freeze. Protect from light. |
| Background / Technical Information: | Flow Cytometry Protocol:- Wash cells in PBS, and resuspend in binding buffer (10mM HEPES/NaOH, pH 7.4, 140mM NaCl, 2.5mM CaCl2; filtered through 0.2µm filter).- Adjust cells to a density between 200’000 and 500’000 cells/ml.- Take 195µl cell suspension and add 5µl Annexin-ATTO 488. Mix and incubate 10 min. in the dark.- Wash cells 1x and resuspend in 190µl binding buffer.- Add 10µl of 20µg/ml propidium iodide (PI) stock solution to stain for necrotic cells.- Analyze by FACS.Note: These are general recommendations. Optimal conditions must be determined individually for each application:Swiss-Prot link P08758: Annexin V (human)AfCS Signalling Gateway link A000281: Annexin V (mouse) |
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