Annexin V (mouse), (recombinant) (Alexa Fluor^® 488 conjugate)



ALX-209-257-T100	100 tests	253.00 USD

Alexa Fluor^® 488-labelled recombinant mouse annexin shows bright green fluorescence (Ex(max): 495nm; Em(max): 519nm).

Product Specification

Quantity:	100 tests/500µl.

Formulation:	Liquid. Contains 0.02% sodium azide.

Specificity:	Binds phosphatidylserine (PS) residues.

Application:	Detection of apoptotic cells by flow cytometry.

Long Term Storage:	+4°C

Handling:	Do not freeze. Protect from light.

Background / Technical Information:	UniProt ID P48036: Annexin V (mouse) AfCS Signaling Gateway ID A000281: Annexin V (mouse)

Protocol:	General Protocol - Detection of apoptotic cells by flow cytometry - Harvest 2x10⁵ cells per staining and wash cells twice in cold phosphate- buffered-saline (PBS). - Resuspend cells in 100µl binding buffer (10mM HEPES, pH 7.4, 140mM sodium chloride, 2.5mM calcium dichloride), add 5µl annexin V-Alexa Fluor^®488 to the cells and mix gently. For detection of necrosis add additionally appropriate amounts of propidium iodide (final concentration 1µg/ml or 1.5µM). - Incubate for 15 min. at room temperature in the dark. - Add 400µl binding buffer and analyze the staining in flow cytometry as soon as possible (within an hour).

Figures: Immature mouse B-cells were treated with Fab₂-antibodies for 24 hours. Stimulated ((B), after anti-IgM cross-linking) and unstimulated premature mouse B-cells ((A) WEHI231) were stained with Annexin-AlexaFluor^®488 as described [1] and analyzed in flow cytometry.

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Product Literature References

Engagement of the antigen-receptor on immature murine B lymphocytes results in death by apoptosis: A. Norvell, et al.; J. Immunol. 154, 4404 (1995), Abstract;