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Annexin V (mouse), (recombinant) (Alexa Fluor® 488 conjugate)

 
ALX-209-257-T100 100 tests 304.00 USD
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Alexa Fluor® 488-labelled recombinant mouse annexin shows bright green fluorescence (Ex(max): 495nm; Em(max): 519nm).

Product Specification

Source:Produced in E. coli.
 
UniProt ID:P48036
 
Gene/Protein Identifier:A000281 (UCSD Signaling Gateway ID)
 
Quantity:100 tests/500µl.
 
Formulation:Liquid. Contains 0.02% sodium azide.
 
Specificity:Binds phosphatidylserine (PS) residues.
 
Applications:Flow Cytometry
 
Application Notes:Detection of apoptotic cells by flow cytometry.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:+4°C
 
Handling:Protect from light. Do not freeze.
 
Protocol:General Protocol - Detection of apoptotic cells by flow cytometry
- Harvest 2x105 cells per staining and wash cells twice in cold phosphate- buffered-saline (PBS).
- Resuspend cells in 100µl binding buffer (10mM HEPES, pH 7.4, 140mM sodium chloride, 2.5mM calcium dichloride), add 5µl annexin V-Alexa Fluor® 488 to the cells and mix gently.
For detection of necrosis add additionally appropriate amounts of propidium iodide (final concentration 1µg/ml or 1.5µM).
- Incubate for 15 min. at room temperature in the dark.
- Add 400µl binding buffer and analyze the staining in flow cytometry as soon as possible (within an hour).
 
209-257
Figures: Immature mouse B-cells were treated with Fab2-antibodies for 24 hours. Stimulated ((B), after anti-IgM cross-linking) and unstimulated premature mouse B-cells ((A) WEHI231) were stained with Annexin-AlexaFluor® 488 as described [1] and analyzed in flow cytometry.
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209-257

Product Literature References

Engagement of the antigen-receptor on immature murine B lymphocytes results in death by apoptosis: A. Norvell, et al.; J. Immunol. 154, 4404 (1995), Abstract;

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