Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

Annexin V (mouse), (recombinant) (Alexa Fluor® 488 conjugate)

ALX-209-257-T100 100 tests 310.00 USD
Do you need bulk/larger quantities?
Alexa Fluor® 488-labelled recombinant mouse annexin shows bright green fluorescence (Ex(max): 495nm; Em(max): 519nm).

Product Specification

Source:Produced in E. coli.
UniProt ID:P48036
Gene/Protein Identifier:A000281 (UCSD Signaling Gateway ID)
Quantity:100 tests/500µl.
Formulation:Liquid. Contains 0.02% sodium azide.
Specificity:Binds phosphatidylserine (PS) residues.
Applications:Flow Cytometry
Application Notes:Detection of apoptotic cells by flow cytometry.
Shipping:Shipped on Blue Ice
Long Term Storage:+4°C
Handling:Protect from light. Do not freeze.
Protocol:General Protocol - Detection of apoptotic cells by flow cytometry
- Harvest 2x105 cells per staining and wash cells twice in cold phosphate- buffered-saline (PBS).
- Resuspend cells in 100µl binding buffer (10mM HEPES, pH 7.4, 140mM sodium chloride, 2.5mM calcium dichloride), add 5µl annexin V-Alexa Fluor® 488 to the cells and mix gently.
For detection of necrosis add additionally appropriate amounts of propidium iodide (final concentration 1µg/ml or 1.5µM).
- Incubate for 15 min. at room temperature in the dark.
- Add 400µl binding buffer and analyze the staining in flow cytometry as soon as possible (within an hour).
Figures: Immature mouse B-cells were treated with Fab2-antibodies for 24 hours. Stimulated ((B), after anti-IgM cross-linking) and unstimulated premature mouse B-cells ((A) WEHI231) were stained with Annexin-AlexaFluor® 488 as described [1] and analyzed in flow cytometry.
Please mouse over

Product Literature References

Engagement of the antigen-receptor on immature murine B lymphocytes results in death by apoptosis: A. Norvell, et al.; J. Immunol. 154, 4404 (1995), Abstract;

Product Toolbox


Certificate of Analysis


By target:
By biological activity:
Annexin Recombinant protein
By catalog section:


Technical Service
Customer Service

Related Literature

Monitor programmed cell death from membrane to nucleus
Monitor programmed cell death from membrane to nucleus
Download as PDF

Technical Posters
Arachidonic Acid Metabolites
Arachidonic Acid Metabolites
Download as PDF

All new literature pieces

Recommend this page

For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2018 Enzo Life Sciences, Inc.,