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ALX-209-256 Revised 05-Mar-10
Annexin V (human), (recombinant) (FITC conjugate)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Annexin V / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-209-256-T100   100 tests 175.00 USD Add To Cart
ALX-209-256-3100   3x100 tests 385.00 USD Add To Cart
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Product Specification
MW: ~52kDa (SDS-PAGE).
QUANTITY: 100 tests/0.5ml; 3x100 tests/3x0.5ml.
PURITY: ≥90%
PURITY DETAIL: Affinity purified.
FORMULATION: Liquid. In PBS, pH 7.4, containing 0.02% sodium azide.
SPECIFICITY: Binds to phosphatidylserine (PS).
APPLICATION: Detection of apoptotic cells by flow cytometry.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
USE/STABILITY: Stable for at least one year after receipt when stored at +4°C.
HANDLING: Do not freeze. Protect from light.
Product Images
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Product Description
FITC-labelled recombinant human annexin V fusion protein (produced in E. coli) shows bright green fluorescence (Ex(max): 488nm; Em(max): 530nm).
General Information
BACKGROUND/TECHNICAL INFORMATION
Flow Cytometry Protocol:

- Wash cells in PBS, and resuspend in binding buffer (10mM HEPES/NaOH, pH 7.4, 140mM NaCl, 2.5mM CaCl2; filtered through 0.2µm filter).
- Adjust cells to a density between 200'000 and 500'000 cells/ml.
- Take 195µl cell suspension and add 5µl Annexin-FITC. Mix and incubate 10 min. in the dark.
- Wash cells 1x and resuspend in 190µl binding buffer.
- Add 10µl of 20µg/ml propidium iodide (PI) stock solution to stain for necrotic cells.
- Analyze by FACS.
Note: These are general recommendations. Optimal conditions must be determined individually for each application:

Swiss-Prot link P08758: Annexin V (human)
AfCS Signalling Gateway link A000281: Annexin V (mouse)
Related Products
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
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