Annexin V (human), (recombinant) (APC conjugate)



ALX-209-252-T020	20 tests	101.00 USD

ALX-209-252-T100	100 tests	288.00 USD

APC-labelled recombinant human annexin V is suitable for dual staining experiments to detect phosphatidylserine whilst cell-surface protein can be detected with a suitable MAb (FITC-labelled or labelled secondary antibody). The fluorochrome APC (allophycocyanin) shows far red fluorescence (Ex(max): 650nm; Em(max): 660nm).

Product Specification

MW:	~35.8kDa.

Quantity:	20 tests/100µl; 100 tests/500µl.

Purity:	≥98% (SDS-PAGE, HPLC)

Formulation:	Liquid. In 50mM TRIS, pH 7.4, containing 100mM sodium chloride, 1% BSA and 0.02% sodium azide.

Source/Host:	Produced in E. coli.

Specificity:	Binds to phosphatidylserine (PS).

Application:	Detection of apoptotic cells by flow cytometry.

Long Term Storage:	+4°C

Handling:	Do not freeze. Protect from light.

Background / Technical Information:	UniProt ID P08758: Annexin V (human) AfCS Signaling Gateway ID A000281 : Annexin V (mouse)

Protocol:	Flow Cytometry Protocol: - Prepare binding buffer (10mM Hepes/NaOH, pH 7.4, 140mM NaCl, 2.5mM CaCl₂; filtered through 0.2µm pore filter); - Brief centrifugation before use is recommended - Wash cells in PBS by gentle shaking or pipetting up and down - resuspend cells in binding buffer; adjust cell density to 2-5x10⁵/ml - Take 195µl cell suspension and add 5µl Annexin V-APC - Mix and incubate 10 min. in the dark at room temperature - Wash cells 1x with PBS and resuspend in 190µl binding buffer - FACS analysis