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Annexin V (human), (recombinant) (APC conjugate)

 
ALX-209-252-T020 20 tests 120.00 USD
 
ALX-209-252-T100 100 tests 347.00 USD
Do you need bulk/larger quantities?
 
APC-labelled recombinant human annexin V is suitable for dual staining experiments to detect phosphatidylserine whilst cell-surface protein can be detected with a suitable MAb (FITC-labelled or labelled secondary antibody). The fluorochrome APC (allophycocyanin) shows far red fluorescence (Ex(max): 650nm; Em(max): 660nm).

Product Specification

MW:~35.8kDa
 
Source:Produced in E. coli.
 
UniProt ID:P08758
 
Quantity:20 tests/100µl; 100 tests/500µl.
 
Formulation:Liquid. In 50mM TRIS, pH 7.4, containing 100mM sodium chloride, 1% BSA and 0.02% sodium azide.
 
Purity:≥89% (SDS-PAGE, HPLC)
 
Specificity:Binds to phosphatidylserine (PS).
 
Applications:Flow Cytometry
 
Application Notes:Detection of apoptotic cells by flow cytometry.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:+4°C
 
Handling:Protect from light. Do not freeze.
 
Protocol:Flow Cytometry Protocol:
- Prepare binding buffer (10mM Hepes/NaOH, pH 7.4, 140mM NaCl, 2.5mM CaCl2; filtered through 0.2µm pore filter);
- Brief centrifugation before use is recommended
- Wash cells in PBS by gentle shaking or pipetting up and down
- Resuspend cells in binding buffer; adjust cell density to 2-5x105/ml
- Take 195µl cell suspension and add 5µl Annexin V-APC
- Mix and incubate 10 min. in the dark at room temperature
- Wash cells 1x with PBS and resuspend in 190µl binding buffer
- FACS analysis
 

Product Literature References

In vitro and in vivo efficacy of non-psychoactive cannabidiol in neuroblastoma: T. Fisher, et al.; Curr. Oncol. 23, S15 (2016), Application(s): Annexin V assay, Abstract; Full Text

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