Annexin V (human), (recombinant) (APC conjugate)
| ALX-209-252-T020 | 20 tests | 106.00 USD |  |
|
| ALX-209-252-T100 | 100 tests | 279.00 USD | |
|
APC-labelled recombinant human annexin V is suitable for dual staining experiments to detect phosphatidylserine whilst cell-surface protein can be detected with a suitable MAb (FITC-labelled or labelled secondary antibody). The fluorochrome APC (allophycocyanin) shows far red fluorescence (Ex(max): 650nm; Em(max): 660nm).
Product Specification
| MW: | ~35.8kDa. |
| Quantity: | 20 tests/100µl; 100 tests/500µl. |
| Purity: | ≥98% (SDS-PAGE, HPLC) |
| Formulation: | Liquid. In 50mM TRIS, pH 7.4, containing 100mM sodium chloride, 1% BSA and 0.02% sodium azide. |
| Source/Host: | Produced in E. coli. |
| Specificity: | Binds to phosphatidylserine (PS). |
| Application: | Detection of apoptotic cells by flow cytometry. |
| Shipping: | SHIPPED ON BLUE ICE |
| Long Term Storage: | +4°C |
| Handling: | Do not freeze. Protect from light. |
| Background / Technical Information: | Flow Cytometry Protocol: - Prepare binding buffer (10mM Hepes/NaOH, pH 7.4, 140mM NaCl, 2.5mM CaCl2; filtered through 0.2µm pore filter); - Brief centrifugation before use is recommended - Wash cells in PBS by gentle shaking or pipetting up and down - resuspend cells in binding buffer; adjust cell density to 2-5x105/ml - Take 195µl cell suspension and add 5µl Annexin V-FITC - Mix and incubate 10 min. in the dark at room temperature - Wash cells 1x with PBS and resuspend in 190µl binding buffer - FACS analysisUniprot link P08758: Annexin V (human)AfCS Signalling Gateway link A000281 : Annexin V (mouse) |
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