FITC-labelled recombinant human annexin V shows bright green fluorescence (Ex(max): 488nm; Em(max): 530nm).
Product Details
| MW: | ~35.8kDa. |
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| Source: | Produced in E. coli. |
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| UniProt ID: | P08758 |
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| Quantity: | 20 tests/100µl; 100 tests/500µl; 300 tests/1'500µl. |
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| Formulation: | Liquid. In 50mM TRIS, pH 7.4, containing 100mM sodium chloride, 1% BSA and 0.02% sodium azide. |
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| Purity: | ≥89% (SDS-PAGE, HPLC) |
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| Specificity: | Binds to phosphatidylserine (PS). |
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| Biological Activity: | Exhibits anti-phospholipase activity. |
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| Applications: | Flow Cytometry
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| Application Notes: | Detection of apoptotic cells by flow cytometry. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | +4°C |
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| Handling: | After opening, prepare aliquots and store at -20°C. |
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| Protocol: | Flow Cytometry Protocol:
- Prepare binding buffer (10mM Hepes/NaOH, pH 7.4, 140mM NaCl, 2.5mM CaCl2; filtered through 0.2µm pore filter).
- Brief centrifugation of annexin V-FITC before use is recommended
- Wash cells in PBS by gentle shaking or pipetting up and down.
- Resuspend cells in binding buffer; adjust cell density to 2-5x105/ml
- Take 195µl cell suspension and add 5µl annexin V-FITC
- Mix and incubate 10 min. in the dark at room temperature
- Wash cells 1x with PBS and resuspend in 190µl binding buffer
- Add 10µl of 20µg/ml propidium iodide stock solution (end concentration 1µg/ml)
- FACS analysis |
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| Regulatory Status: | RUO - Research Use Only |
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