Calmodulin (wheat)



ALX-204-005-C250	250 µg	48.00 USD

ALX-204-005-M001	1 mg	130.00 USD

Wheat germ calmodulin sequence is unique in that it contains cysteine (Cys²⁶) and only a single tyrosine (Tyr¹³⁸), in contrast to mammalian CaMs, which have two tyrosine residues and lack cysteine. This allows wheat germ CaM to be independently derivatized at both N- and C-terminal regions. Therefore it is ideal for applications requiring both calmodulin activation and residue specific tagging. Wheat germ CaM also has no tryptophan resulting in a distinctive absorbance spectrum when compared to mammalian CaM sequences.

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Product Specification

Alternative Name:	Phosphodiesterase 3':5'-cyclic nucleotide activator, CaM

MW:	~17kDa (SDS-PAGE)

Purity:	≥98% (SDS-PAGE)

Formulation:	Lyophilized from 5% sucrose, pH 7.0, containing 10mM sodium phosphate.

Specific Activity:	≥35’000U/mg protein. One unit will stimulate 0.01 unit of 3’:5’-cyclic nucleotide phosphodiesterase in a 3ml reaction volume at pH 7.5 and 30°C, to 50% of the maximum activity of the enzyme when saturated with CaM in the presence of 100µM Ca²⁺.

MI:	14: 1719

Source/Host:	Isolated from wheat germ (Triticum aestivum).

Reconstitution:	Reconstitute with 1ml distilled water.

Application:	Can be independently derivatized at both N- and C-terminal regions within the molecule for structural, proteomic biomarker discovery and protein-protein interaction studies.

Long Term Storage:	-20°C

Handling:	Avoid freeze/thaw cycles. Protect from light.

Background / Technical Information:	UniProt ID P04464: Calmodulin (wheat)

Product Literature References

Site-specific derivatives of wheat germ calmodulin. Interactions with troponin and sarcoplasmic reticulum: G.M. Strasburg, et al.; J. Biol. Chem. 263, 542 (1988), Abstract; Full Text

General Literature References

A rapid and sensitive method for detection and quantification of calcineurin and calmodulin-binding proteins using biotinylated calmodulin: M.L. Billingsley, et al.; PNAS 82, 7585 (1985), Abstract; Full Text