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Control:Fc fusion protein (human), (recombinant)

Negative control for Fc chimeric proteins
 
ALX-203-004-C050 50 µg 425.00 USD
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Negative control for Fc chimeric proteins. Does not bind to a series of ligands in an ELISA assay. Does not inhibit binding between the thymoma cell line EL-4 expressing β3-integrin and astrocytes expressing high level of Thy-1 in mice.

Product Specification

Alternative Name:Thy-1 (RLE mutant)
 
MW: ~ 53 kDa
 
Source:Produced in HEK 293 cells. The extracellular domain of mutated hThy-1, hThy-1 (RLE) (aa 20-130), is fused to the Fc portion of human IgG1. The integrin binding sequence RLD of hThy-1 has been changed to RLE abolishing the binding of integrins.
 
Formulation:Lyophilized. Contains PBS.
 
Purity:≥90% (SDS-PAGE)
 
Purity Detail:Purified by multi-step chromatography.
 
Specificity:Does not bind to β3-integrin nor to any of a large number of tested TNF-superfamily members including rhsTRAIL, rhsCD40L, rhsAPRIL, etc.
 
Application Notes:Control compound for Fc Tagged Proteins, ELISA, Flow Cytometry, Functional Application (serves as a negative control in functional assays of TNF-superfamily members).
 
Reconstitution:Reconstitute with 50µl sterile distilled water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least 6 months after receipt when stored as recommended.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
 
Scientific Background:Thy-1 is a glycosyl phosphatidylinositol (GPI)- anchored glycoprotein of the immunoglobulin superfamily expressed in various cell types, particularly in T-lymphocytes and cells of the neuronal system. Thy-1 is involved in cell adhesion, and the presence of a RLD motifs, which is recognized by intengrins, strengthen the hypothesis that the Thy-1:integrin interaction is responsible for cell attachment. In astrocytes, binding of Thy-1 to β3-integrin triggers tyrosine phosphorylation of focal adhesion proteins.
 
Control:Fc fusion protein (human), (recombinant) SDS-PAGE
SDS-PAGE Analysis. Lane 1: MW Marker, Lane 2: 1 µg Fc:control protein
Control:Fc fusion protein (human), (recombinant) ELISA analysis
ELISA analysis of Control:Fc fusion protein (human), (recombinant), (Prod. No. ALX-203-004): Negative binding to members in TNF-superfamily, including, but not limited to, CD40L, TRAIL, TNF, CD137L, FasL at various concentrations as indicated. (The CD40L and TRAIL used in the assay are fully capable to bind to their native biological receptors , i.e. CD40 and TRAIL-R2, respectively, as shown by the red square and diamond.)
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Control:Fc fusion protein (human), (recombinant) SDS-PAGE Control:Fc fusion protein (human), (recombinant) ELISA analysis

Product Literature References

CD40 is a major regulator of dendrite growth from developing excitatory and inhibitory neurons: P. Carriba & A.M. Davies; Elife 6, e30442 (2017), Abstract; Full Text
Pro-inflammatory effects of interleukin-35 in rheumatoid arthritis: M. Filková, et al.; Cytokine 73, 36 (2015), Application(s): Cell Culture: IHC, IF used to analyze IL-35 in PBMCs, Abstract;
Semaphorin 3C is a novel adipokine linked to extracellular matrix composition: N. Mejhert, et al.; Diabetologia. 56, 1792 (2013), Abstract;
IL-33 promotes the migration and proliferation of circulating fibrocytes from patients with allergen-exacerbated asthma: L. Bianchetti, et al.; Biochem. Biophys. Res. Commun. 426, 116 (2012), Abstract;
Induction of GITRL expression in human keratinocytes by Th2 cytokines and TNF‐α: implications for atopic dermatitis: A.M. Byrne, et al.; Clin. Exp. Allergy. 42, 550 (2012), Abstract; Full Text
Lymphotoxin-mediated regulation of gammadelta cell differentiation by alphabeta T cell progenitors: B. Silva-Santos, et al.; Science 307, 925 (2005), Abstract;
The role of junctional adhesion molecule-C (JAM-C) in oxidized LDL-mediated leukocyte recruitment: T. Keiper, et al.; FASEB J. 19, 2078 (2005), Abstract; Full Text

General Literature References

Signaling triggered by Thy-1 interaction with beta 3 integrin on astrocytes is an essential step towards unraveling neuronal Thy-1 function: A.M. Avalos, et al.; Biol. Res. 35, 231 (2002), Abstract;
Thy-1 binds to integrin beta(3) on astrocytes and triggers formation of focal contact sites: L. Leyton, et al.; Curr. Biol. 11, 1028 (2001), Abstract;

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