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Control:Fc fusion protein (human), (recombinant)

Negative control for Fc chimeric proteins
 
ALX-203-004-C050 50 µg 405.00 USD
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Negative control for Fc chimeric proteins. Does not bind to a series of ligands in an ELISA assay. Does not inhibit binding between the thymoma cell line EL-4 expressing β3-integrin and astrocytes expressing high level of Thy-1 in mice.

Product Specification

Alternative Name:Thy-1 (RLE mutant)
 
MW: ~ 53 kDa
 
Source:Produced in HEK 293 cells. The extracellular domain of mutated hThy-1, hThy-1 (RLE) (aa 20-130), is fused to the Fc portion of human IgG1. The integrin binding sequence RLD of hThy-1 has been changed to RLE abolishing the binding of integrins.
 
Formulation:Lyophilized. Contains PBS.
 
Purity:≥90% (SDS-PAGE)
 
Purity Detail:Purified by multi-step chromatography.
 
Specificity:Does not bind to β3-integrin nor to any of a large number of tested TNF-superfamily members including rhsTRAIL, rhsCD40L, rhsAPRIL, etc.
 
Application Notes:Control compound for Fc Tagged Proteins, ELISA, Flow Cytometry, Functional Application (serves as a negative control in functional assays of TNF-superfamily members).
 
Reconstitution:Reconstitute with 50µl sterile distilled water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least 6 months after receipt when stored as recommended.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
 
Scientific Background:Thy-1 is a glycosyl phosphatidylinositol (GPI)- anchored glycoprotein of the immunoglobulin superfamily expressed in various cell types, particularly in T-lymphocytes and cells of the neuronal system. Thy-1 is involved in cell adhesion, and the presence of a RLD motifs, which is recognized by intengrins, strengthen the hypothesis that the Thy-1:integrin interaction is responsible for cell attachment. In astrocytes, binding of Thy-1 to β3-integrin triggers tyrosine phosphorylation of focal adhesion proteins.
 

ALX-203-004 SDS-PAGE
SDS-PAGE Analysis. Lane 1: MW Marker, Lane 2: 1 µg Fc:control protein
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ALX-203-004 SDS-PAGE

Product Literature References

Pro-inflammatory effects of interleukin-35 in rheumatoid arthritis: M. Filková, et al.; Cytokine 73, 36 (2015), Application(s): Cell Culture: IHC, IF used to analyze IL-35 in PBMCs, Abstract;
Semaphorin 3C is a novel adipokine linked to extracellular matrix composition: N. Mejhert, et al.; Diabetologia. 56, 1792 (2013), Abstract;
IL-33 promotes the migration and proliferation of circulating fibrocytes from patients with allergen-exacerbated asthma: L. Bianchetti, et al.; Biochem. Biophys. Res. Commun. 426, 116 (2012), Abstract;
Induction of GITRL expression in human keratinocytes by Th2 cytokines and TNF‐α: implications for atopic dermatitis: A.M. Byrne, et al.; Clin. Exp. Allergy. 42, 550 (2012), Abstract; Full Text
Lymphotoxin-mediated regulation of gammadelta cell differentiation by alphabeta T cell progenitors: B. Silva-Santos, et al.; Science 307, 925 (2005), Abstract;
The role of junctional adhesion molecule-C (JAM-C) in oxidized LDL-mediated leukocyte recruitment: T. Keiper, et al.; FASEB J. 19, 2078 (2005), Abstract; Full Text

General Literature References

Signaling triggered by Thy-1 interaction with beta 3 integrin on astrocytes is an essential step towards unraveling neuronal Thy-1 function: A.M. Avalos, et al.; Biol. Res. 35, 231 (2002), Abstract;
Thy-1 binds to integrin beta(3) on astrocytes and triggers formation of focal contact sites: L. Leyton, et al.; Curr. Biol. 11, 1028 (2001), Abstract;

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