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ACRP30headless (mouse), (recombinant)

 
ALX-203-003-C010 10 µg 275.00 USD
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Control compound for rmsMEGACD40L® (Prod. No. ALX-522-120).

Product Specification

Alternative Name:Tail-Adp, Tail Adiponectin, Adipocyte complement related protein of 30kDa headless
 
MW:~25kDa
 
Source:Produced in HEK 293 cells. The collagen domain of mouse ACRP30 (aa 18-111) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG®-tag.
 
UniProt ID:Q60994
 
Formulation:Lyophilized. Contains PBS.
 
Purity:≥90% (SDS-PAGE)
 
Purity Detail:Purified by multi-step chromatography.
 
Endotoxin Content:<0.1EU/µg protein (LAL-test).
 
Application Notes:Negative control for fusion proteins containing mouse ACRPheadless.
 
Reconstitution:Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least 6 months after receipt when stored as recommended.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
 
Technical Info/Product Notes:FLAG is a registered trademark of Sigma-Aldrich Co.
 
203-003
Figure: ACRP30headless (mouse) does not bind to rhTNF-R1 and rhOX40:Fc in an ELISA assay.
Method: A 96 well ELISA plate was coated O/N at RT with 100 µl of 1 µg/ml rhTNF-R1:Fc (Prod. No. ALX-522-013) and 1 µg/ml rhOX40:Fc (Prod. No. ALX-522-022) in PBS. 100 µl of rmsACRP30headless at 8 µg/ml were mixed with 100 µl 0.5% FCS in PBS in the first well and a series of 1:1 dilutions was prepared for the following wells. Binding of rmsACRP30headless to the receptor:Fc was detected after 1 hr incubation at 37°C using a goat anti-human HRP conjugated antibody (Jackson lab; 1:1’000 dilution, 1 hr incubation). O-phenylenediamine dihydrochloride (OPD) was used as a substrate, and absorbance was measured at 490 nm in an ELISA reader.
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203-003

Related Products

MEGACD40L® (soluble) (mouse), (recombinant) 

Highly active
Produced in CHO cells. The extracellular domain of mouse CD40L (CD154) (aa 115-260) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag., ≥95% (SDS-PAGE) | Print as PDF
 
ALX-522-120-C010 10 µg 525.00 USD
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