ACRP30headless (human), (recombinant)



ALX-203-002-C010	10 µg	255.00 USD

Product Specification

Alternative Name:	Adipocyte complement related protein of 30kDa headless, Tail-Adp, Tail Adiponectin

Concentration:	0.1mg/ml after reconstitution.

Endotoxin Content:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).

MW:	~27kDa (SDS-PAGE).

Purity:	≥95% (SDS-PAGE)

Formulation:	Lyophilized. Contains PBS.

Source/Host:	Produced in HEK 293 cells. The non-homologous (aa 16-45) and collagen domain of human ACRP30 (aa 45-108) are fused at the N-terminus to a FLAG^®-tag.

Reconstitution:	Reconstitute with 100µl sterile water.

Application:	Negative control. When used at 1µg/g body weight in mouse demonstrated no significant bioactivity in terms of reduction of serum glucose compared to wildtype in an in vivo assay. Control compound for rhsMegaAPRIL™ (Prod. No. ALX-522-035) and rhsMegaTNF-α (Prod. No. ALX-522-046).

Long Term Storage:	-20°C

Use/Stability:	Stable for at least 6 months after receipt when stored as recommended.

Handling:	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Background / Technical Information:	UniProt ID Q15848: ACRP30 (human) FLAG is a registered trademark of Sigma-Aldrich Co.

Figure 1: Bioactivity of rmsAdiponectin.Method: 2 groups of 4 mice each were injected with PBS (vehicle), rmAdiponectin (Prod. No. ALX-522-059) and rhACRP30headless (tail adiponectin) (Prod. No. ALX-203-002) at 1µg/g body weight as a tail vein injection. Subsequently, serum glucose levels were monitored over the time indicated by Trinder assay. Plotted is the average of 4 mice. rmAdiponectin represses hepatic glucose production, so serum glucose levels decrease until counter-regulatory mechanisms (i.e. glucagon) become effective. rhACRP30headless demonstrated no significant bioactivity in terms of reduction of serum glucose compared to wildtype in this in vivo assay.

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Product Literature References

Adiponectin acts in the brain to decrease body weight: Y. Qi, et al.; Nat. Med. 10, 524 (2004), Abstract;

Structure-function studies of the adipocyte-secreted hormone Acrp30/adiponectin: Implications for metabolic regulation and bioactivity: U.B. Pajvani, et al.; J. Biol. Chem. 278, 9073 (2003), Abstract; Full Text