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PARG (bovine thymus)

 
ALX-202-045-UC01 0.1 U 448.00 USD
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Replaces Prod. #: BML-SE179

Product Specification

Alternative Name:Poly(ADP-ribose) glycohydrolase
 
Source:Isolated from calf thymus.
 
UniProt ID:O02776
 
Formulation:Liquid. In 50mM K-PO4 buffer, pH 7.5, containing 50mM potassium chloride, 0.1% Triton-x 100, 10% glycerol and 1mM DTT.
Note: add 10mM β-Mercaptoethanol.
 
Purity Detail:Purified by substrate chromatography. The enzyme is composed of two major bands at 74kDa and 59kDa.
 
Specific Activity:~20’000U/mg protein. 1U is defined as the amount of enzyme that hydrolyzes 1nmol of poly(ADP-ribose) per min. at 37°C.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Use/Stability:For further dilution use the following enzyme dilution buffer: 50mM KPO4, pH 7.5, 50mM KCl, 10 mM β-mercaptoethanol, 10% glycerol, 1mM DTT, 0.1% Triton.
 
Handling:Avoid freeze/thaw cycles.
 

Product Literature References

Methods for biochemical study of poly(ADP-ribose) metabolism in vitro and in vivo: G.M. Shah et al.; Anal. Biochem. 227, 1 (1995), Abstract;
Mode of action of poly(ADP-ribose) glycohydrolase: G. Brochu, et al.; Biochim. Biophys. Acta 1219, 342 (1994), Abstract;
Purification of poly(ADP-ribose) glycohydrolase and detection of its isoforms by a zymogram following one- or two-dimensional electrophoresis: G. Brochu et al.; Anal. Biochem 218, 265 (1994), Abstract;
Poly(ADP-ribosyl)ation of chromatin in an in vitro poly(ADP-ribose)-turnover system: H. Thomassin, et al.; Biochim. Biophys. Acta 1137, 171 (1992), Abstract;
An affinity matrix for the purification of poly(ADP-ribose) glycohydrolase: H. Thomassin, et al.; Nucl. Acid Res. 18, 4691 (1990), Abstract;
Rapid assay of poly(ADP-ribose) glycohydrolase: L. Menard & G.G. Poirier; Biochem. Cell Biol. 65, 668 (1987), Abstract;

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The PARP Family & ADP Ribosylation
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