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Poly(ADP-ribose), standard

 
ALX-202-043-C001 1 µg 346.00 USD
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Product Specification

Alternative Name:PAR
 
MW:1’100 to 275’000 depending on the number of units of ADP-ribose.
 
Source:Released from automodified PARP-1 by base treatment and purified by boronate affinity.
 
Quantity:1µg representing 1.8 nanomoles of 2-300 units of ADP-ribose.
 
Formulation:Liquid. In water.
 
Purity:≥95% (HPLC of ADP-ribose RNA and DNA measurement)
 
Application Notes:Immunoblotting standard for the detection of poly(ADP-ribose) (PAR) of 3-200 bases in size sythesized in vitro or in vivo.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Use/Stability:Stable for one week when stored at +4°C. Stable for at least 2 years after receipt when stored at -80°C.
 
Handling:Avoid freeze/thaw cycles.
 

Product Literature References

Immunological determination and size characterization of poly(ADP- ribose) synthesized in vitro and in vivo: E.B. Affar, et al.; Biochim. Biophys. Acta 1428, 137 (1999), Abstract;
Poly(ADP-ribose) turnover in quail myoblast cells: relation between the polymer level and its catabolism by glycohydrolase: E.B. Affar, et al.; Mol. Cell. Biochem. 193, 127 (1999), Abstract;
Immunodot blot method for the detection of poly(ADP-ribose) synthesized in vitro and in vivo: E.B. Affar, et al.; Anal. Biochem. 259, 280 (1998), Abstract;
Methods for biochemical study of poly(ADP-ribose) metabolism in vitro and in vivo.: G.M. Shah, et al.; Anal. Biochem. 227, 1 (1995), Abstract;
Rapid assay of poly(ADP-ribose) glycohydrolase: L. Menard & G.G. Poirier; Biochem. Cell Biol. 65, 668 (1987), Abstract;

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The PARP Family & ADP Ribosylation
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